Mouse Renin 1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse Renin 1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF4277-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF4277-SP
别名	angiotensin-forming enzyme; Angiotensinogenase; EC 3.4.23; EC 3.4.23.15; FLJ10761; HNFJ2; REN; renin precursor, renal; renin
反应种属	Mouse
应用	Western Blot,Immunohistochemistry,Immunoprecipitation,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard
目标/特异性	Detects mouse Renin 1 in ELISAs and Western blots. In sandwich immunoassays, approximately 5% cross-reactivity with recombinant rat Renin is observed, and less than 0.2% cross-reactivity with recombinant human Renin is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard :
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5972 (Human); 19701 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Transcriptome-based analysis of kidney gene expression changes associated with diabetes in OVE26 mice, in the presence and absence of losartan treatment. Authors: Komers R, Xu B, Fu Y, McClelland A, Kantharidis P, Mittal A, Cohen H, Cohen D PLoS ONE, 2014;9(5):e96987. Species: Mouse Sample Type: Tissue Homgenates Application: WB Chicken Ovalbumin Upstream Promoter Transcription Factor II Regulates Renin Gene Expression. Authors: Mayer S, Roeser M, Lachmann P J. Biol. Chem., 2012;287(29):24483-91. Species: Mouse Sample Type: Tissue Homogenates Application: WB Intrarenal renin angiotensin system revisited: role of megalin-dependent endocytosis along the proximal nephron. Authors: Pohl M, Kaminski H, Castrop H, Bader M, Himmerkus N, Bleich M, Bachmann S, Theilig F J. Biol. Chem., 2010;285(53):41935-46. Species: Mouse Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse Renin 1 Leu22-Arg402 Accession # P06281
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Mouse Renin is a secreted, 42‑47 kDa glycosylated member of the peptidase A1 family. It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I. In mouse, there are two genes that code for Renin. One is in the submandibular gland and the other is in the kidney. The two mature Renin molecules are 95% amino acid (aa) identical. Renal Renin (Renin 1) is synthesized as a 381 aa proform (aa 22‑402). In the kidney, pro‑Renin is proteolytically cleaved after Thr71 to generate a mature enzyme. Mouse pro Renin shares 70% and 85% aa sequence identity with human and rat pro Renin, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	ICC / IHC Images: Renin New Tools: Recombinant Proteases of the Renin-Angiotensin System (RAS) Protein/Peptide Hormones and Regulators Renal Markers Renin-Angiotensin System

参考图片

Renin in Mouse Kidney. Renin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Renin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4277) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glomeruli and tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.