Mouse TNF RII/TNFRSF1B Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse TNF RII/TNFRSF1B Affinity Purified Polyclonal Ab (25 UG)

货号： AF-426-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-426-SP
别名	CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNF RII; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II
全称	Tumor Necrosis Factor Receptor II
反应种属	Mouse
应用	Western Blot,Flow Cytometry,CyTOF-ready,Immunocytochemistry
目标/特异性	Detects mouse TNF RII in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TNF RII is observed and less than 1% cross-reactivity with recombinant mouse (rm) CD27, rmGITR, rhDR6, rmCD30, rmCD40, rmEDAR, rmFas, rmOPG, rmRANK, and rmTNF RI is observed.
使用方法	Western Blot: 0.2 µg/mL Flow Cytometry: 2.5 µg/10⁶cells CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7133 (Human); 21938 (Mouse); 156767 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Endogenous TNF? orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation Authors: S Arokiasamy, C Zakian, J Dilliway, W Wang, S Nourshargh, MB Voisin Sci Rep, 2017;7(0):44189. Species: Mouse Sample Type: Whole Cells Application: Flow Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2 Authors: R Fischer, J Marsal, C Guttà, SA Eisler, N Peters, JR Bethea, K Pfizenmaie, RE Kontermann Sci Rep, 2017;7(1):6607. Species: Mouse Sample Type: Whole Cells Phenotypic screening reveals TNFR2 as a promising target for cancer immunotherapy Oncotarget, 2016;0(0):. Species: Mouse Sample Type: Whole Cells Application: Flow Myosin light chain kinase expression induced via tumor necrosis factor receptor 2 signaling in the epithelial cells regulates the development of colitis-associated carcinogenesis. Authors: Suzuki M, Nagaishi T, Yamazaki M, Onizawa M, Watabe T, Sakamaki Y, Ichinose S, Totsuka M, Oshima S, Okamoto R, Shimonaka M, Yagita H, Nakamura T, Watanabe M PLoS ONE, 2014;9(2):e88369. Species: Mouse Sample Type: Cell Lysates Application: WB Therapy of peritoneally disseminated colon cancer by TAP-deficient embryonic stem cell-derived macrophages in allogeneic recipients. Authors: Haga E, Endo Y, Haruta M, Koba C, Matsumura K, Takamatsu K, Ikeda T, Nishimura Y, Senju S J Immunol, 2014;193(4):2024-33. Species: Mouse Sample Type: Whole Cells Application: Flow Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo. Authors: Proebstl D, Voisin MB, Woodfin A, Whiteford J, DAcquisto F, Jones GE, Rowe D, Nourshargh S J. Exp. Med., 2012;209(6):1219-34. Species: Mouse Sample Type: Whole Tissue Application: IHC TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice. Authors: Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM J. Clin. Invest., 2009;119(10):2954-64. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse TNF RII/TNFRSF1B Val23-Gly258 Accession # P25119
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Two types of soluble TNF receptors have been identified in human serum and urine which can neutralize the biological activities of TNF-alpha and TNF-beta. These binding proteins represent truncated forms of the two types of high-affinity cell surface receptors for TNF (TNFR-p60 Type B and TNFR-p80 Type A). Soluble TNF RII corresponds to TNFR-p80 Type A. In the new TNF superfamily nomenclature, TNF RII is referred to as TNFRSF1B. These apparent soluble forms of the receptors appear to arise as a result of shedding of the extracellular domains of the membrane-bound receptors. Normal concentrations as high as 4 ng/mL are found in the serum of healthy individuals, and even higher levels may be found in some pathological conditions. Although the physiological role of these proteins is not known, it has been speculated that shedding of the soluble receptors in response to TNF release could serve as a mechanism to scavenge the TNF not immediately bound and thus localize the inflammatory response. It is also possible that the pool of TNF bound to soluble receptors could represent a reservoir for the controlled release of TNF.
运输条件	Blue Ice
存放说明	4℃
参考文献	Adipocytokines Cell Structure and Signaling Molecules Co-stimulatory and Co-inhibitory Molecules Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines in Lipid/Cholesterol Metabolism Diabetes Diabetic Peripheral Neuropathy Endothelial Cell Markers Neuroinflammation Obesity Platelets Regulation of Apoptosis by TNF Superfamily Members Regulation of Natural Killer Cell Co-stimulation by TNF Superfamily Members Regulation of T Cell Co-stimulation by TNF Superfamily Members Regulatory T Cells (Tregs) T Cell Cytokine Signaling TNF Superfamily TNF Superfamily Receptors

参考图片

Detection of Mouse TNF RII/TNFRSF1B by Western Blot.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 1 µg/mL LPS for 24 hours. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TNF RII/TNFRSF1B at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of TNF RII/TNFRSF1B in L‑929 Mouse Cell Line by Flow Cytometry.

L‑929 mouse fibroblast cell line was stained with Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).