Mouse TNF RI/TNFRSF1A Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse TNF RI/TNFRSF1A Affinity Purified Polyclonal Ab (25 UG)

货号： AF-425-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-425-SP
别名	CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor
全称	Tumor Necrosis Factor Receptor I
反应种属	Mouse
应用	Western Blot,Flow Cytometry,Immunohistochemistry,Agonist Activity,CyTOF-ready
目标/特异性	Detects mouse TNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 5% cross-reactivity with recombinant human TNF RI is observed.
使用方法	Western Blot: 0.2 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL Agonist Activity: Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is typically 0.05-0.15 μg/mL. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Endogenous TNF? orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation Authors: S Arokiasamy, C Zakian, J Dilliway, W Wang, S Nourshargh, MB Voisin Sci Rep, 2017;7(0):44189. Species: Mouse Sample Type: Whole Cells Application: Flow Sorafenib tosylate inhibits directly necrosome complex formation and protects in mouse models of inflammation and tissue injury Authors: S Martens, M Jeong, W Tonnus, F Feldmann, S Hofmans, V Goossens, N Takahashi, JH Bräsen, EW Lee, P Van der Ve, J Joossens, K Augustyns, S Fulda, A Linkermann, J Song, P Vandenabee Cell Death Dis, 2017;8(6):e2904. Species: Mouse Sample Type: Cell Lysates Application: IP Anti-inflammatory cytokines directly inhibit innate but not adaptive CD8+ T cell functions. Authors: Freeman B, Meyer C, Slifka M J Virol, 2014;88(13):7474-84. Species: Mouse Sample Type: Whole Cells Application: Flow Transforming growth factor beta-activated kinase 1 signaling pathway critically regulates myocardial survival and remodeling. Authors: Li L, Chen Y, Doan J, Murray J, Molkentin J, Liu Q Circulation, 2014;130(24):2162-72. Species: Mouse Sample Type: Cell Lysates Application: WB Therapy of peritoneally disseminated colon cancer by TAP-deficient embryonic stem cell-derived macrophages in allogeneic recipients. Authors: Haga E, Endo Y, Haruta M, Koba C, Matsumura K, Takamatsu K, Ikeda T, Nishimura Y, Senju S J Immunol, 2014;193(4):2024-33. Species: Mouse Sample Type: Whole Cells Application: Flow Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo. Authors: Proebstl D, Voisin MB, Woodfin A, Whiteford J, DAcquisto F, Jones GE, Rowe D, Nourshargh S J. Exp. Med., 2012;209(6):1219-34. Species: Mouse Sample Type: Whole Tissue Application: IHC TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice. Authors: Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM J. Clin. Invest., 2009;119(10):2954-64. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen The function of TRADD in signaling through tumor necrosis factor receptor 1 and TRIF-dependent Toll-like receptors. Authors: Pobezinskaya YL, Kim YS, Choksi S, Morgan MJ, Li T, Liu C, Liu Z Nat. Immunol., 2008;9(9):1047-54. Species: Mouse Sample Type: Cell Lysates Application: IP P2Y1 receptor-evoked glutamate exocytosis from astrocytes: control by tumor necrosis factor-alpha and prostaglandins. Authors: Domercq M, Brambilla L, Pilati E, Marchaland J, Volterra A, Bezzi P J. Biol. Chem., 2006;281(41):30684-96. Species: Rat Sample Type: Whole Cells Application: ICC Thyrocytes isolated from autoimmune-diseased thyroids secrete soluble tumor necrosis factor-R1 that is related to their elevated protein kinase C activity. Authors: LaBue M, Colburn KK, Green LM Thyroid, 2004;14(4):249-62. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse TNF RI Ile22-Ala212 Accession # P25118
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is typically 0.05-0.15 μg/mL.
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	TNF receptor 1 (TNF RI; also called TNF R‑p55/p60, TNFRSF1A and CD120a) is a type I transmembrane protein that belongs to the TNF receptor superfamily (1, 2). TNF RI is widely expressed and is present on the cell surface as a trimer of 55 kDa subunits. It serves as a receptor for both TNF‑ alpha and TNF‑ beta /lymphotoxin. Each subunit contains four TNF‑ alpha trimer‑binding cysteine‑rich domains (CRD) in its extracellular domain (ECD) (1‑6). TNF‑ alpha binding to TNF R1 induces the sequestration of TNFRI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM‑17/TACE (7, 8). Release of the 28‑34 kDa TNF RI ECD occurs constitutively, and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 7‑12). Full‑length TNF RI may also be released in exosome‑like vesicles (12). Such release helps to resolve inflammatory reactions as it down‑regulates cell surface TNF RI and provides soluble TNF RI to bind TNF‑ alpha (6, 13, 14). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (7, 15). Although there is a second receptor for TNF‑ alpha (TNF R2), TNF RI is thought to mediate most of the cellular effects of TNF‑ alpha (3). TNF R1 is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria monocytogenes (1‑3). Mouse TNF RI is a 454 amino acid (aa) protein that contains a 21 aa signal sequence and a 191 aa ECD with a PLAD domain (6). This mediates constitutive trimer formation. The PLAD domain is followed by four CRDs, a 23 aa transmembrane domain, and a 219 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (16). The ECD of mouse TNF RI shows 67%, 70%, 64%, 70% and 88% aa identity with canine, feline, procine, human and rat TNF RI, respectively; and it shows 23% aa identity with the ECD of TNF RII.
运输条件	Blue Ice
存放说明	4℃
参考文献	Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1. Peschon, J.J. et al. (1998) J. Immunol. 160:943. Banner, D.W et al. (1993) Cell 73:431. Medvedev, A.E. et al. (1996) J. Biol. Chem. 271:9778. Chan, F.K. et al. (2000) Science 288:2351. Legler, D.F. et al. (2003) Immunity 18:655. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367. Jin, L. et al. (2000) J. Immunol. 165:5153. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190. Islam, A. et al. (2006) J. Biol. Chem. 281:6860. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105. McDermott, M.F. et al. (1999) Cell 97:133. Schneider-Brachert, W. et al. (2004) Immunity 21:415. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.

参考图片

Detection of TNF RI/TNFRSF1A in L‑929 Mouse Cell Line by Flow Cytometry. L‑929 mouse fibroblast cell line was stained with Goat Anti‑Mouse TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-425-PB, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Detection of Mouse TNF RI/TNFRSF1A by Western Blot. Western blot shows lysates of L‑929 mouse fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-425-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TNF RI/TNFRSF1A at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.