Mouse FGF-8b Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse FGF-8b Affinity Purified Polyclonal Ab (25 UG)

货号： AF-423-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-423-SP
别名	AIGF; AIGFKAL6; Androgen-induced growth factor; FGF8; FGF-8; fibroblast growth factor 8 (androgen-induced); fibroblast growth factor 8; HBGF-8; Heparin-binding growth factor 8; MGC149376
全称	Fibroblast Growth Factor 8
反应种属	Mouse
应用	Western Blot,Immunohistochemistry,Neutralization
目标/特异性	Detects mouse FGF‑8b Isoform in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) FGF acidic, rhFGF basic, rhFGF-4, rhFGF-5, rhFGF-6, rhFGF-7 and rhFGF-9 is observed. It does, however, cross‑react with recombinant mouse FGF-8c in neutralizing bioassay.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize FGF‑8-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND₅₀) is typically 0.4-2.4 µg/mL in the presence of 60 ng/mL Recombinant Mouse FGF‑8b Isoform and 1 µg/mL heparin.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2253 (Human); 14179 (Mouse); 29349 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Fgf20 is required for sensory epithelial specification in the developing cochlea. Authors: Hayashi T, Ray CA, Bermingham-McDonogh O J. Neurosci., 2008;28(23):5991-9. Species: Mouse Sample Type: Whole Cells Application: Neut Cardiac arterial pole alignment is sensitive to FGF8 signaling in the pharynx. Authors: Hutson MR, Zhang P, Stadt HA, Sato AK, Li YX, Burch J, Creazzo TL, Kirby ML Dev. Biol., 2006;295(2):486-97. Species: Chicken Sample Type: Whole Tissue Application: Neut FGF8 is required for cell survival at distinct stages of nephrogenesis and for regulation of gene expression in nascent nephrons. Authors: Grieshammer U, Cebrian C, Ilagan R, Meyers E, Herzlinger D, Martin GR Development, 2005;132(17):3847-57. Species: Mouse Sample Type: Whole Tissue Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse FGF-8b Gln23-Arg215 Accession # NP_006110
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	FGF-8 is a member of the fibroblast growth factor family that was originally discovered as a growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells (1‑3). Alternate splicing of mouse FGF-8 mRNA generates eight secreted isoforms, designated a‑h, but only FGF-8a, b, e and f exist in humans (4). FGF-8 contains a 22 amino acid (aa) signal sequence, an N‑terminal domain that varies according to the isoform (30 aa for FGF-8b; 20 aa for the shortest, FGF-8a), a 125 aa FGF domain and a 37 aa proline‑rich C‑terminal sequence. The FGF domain of FGF-8 shares the most aa identity with FGF17 (75%) and FGF-18 (67%), and the three form an FGF subfamily (2). Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It plays an organizing and inducing role during gastrulation, and regulates patterning of the midbrain/hindbrain, eye, ear, limbs and heart in the embryo (2, 5‑8). The isoforms may play different roles in development. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity although FGF-8a and FGF-8e are also transforming and have been found in human prostate, breast or ovarian tumors (1, 5, 9‑12). FGF-8 shows limited expression in the normal adult, but low levels are found in the reproductive and genitourinary tract, peripheral leukocytes and bone marrow hematopoietic cells (3, 9, 13).
运输条件	Blue Ice
存放说明	4℃
参考文献	Mattila, M.M. and P.L. Harkonen (2007) Cytokine Growth Factor Rev. 18:257. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139. Tanaka, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8928. Gemel, J. et al. (1996) Genomics 35:253. Olsen, S.K. et al. (2006) Genes Dev. 20:185. Crossley, P.H. et al. (1996) Cell, 84:127. Heikinheimo, M. et al. (1994) Mech. Dev. 48:129. Sun, X. et al. (1999) Genes Dev. 13:1834. Ghosh, A.K. et al. (1996) Cell Growth Differ. 7:1425. Mattila, M.M. et al. (2001) Oncogene 20:2791. Valve, E. et al. (2000) Int. J. Cancer 88:718. Valve, E.M. et al. (2001) Lab. Invest. 81:815. Nezu, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:843.

参考图片

FGF‑8 in Human Prostate. FGF‑8 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Mouse FGF‑8 b Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-423-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial and endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Cell Proliferation Induced by FGF‑8 and Neutralization by Mouse FGF‑8 Antibody. Recombinant Mouse FGF‑8b Isoform (Catalog # 423-F8) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse FGF‑8b Isoform (60 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse FGF‑8b Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-423-NA). The ND₅₀ is typically 0.4-2.4 µg/mL in the presence of heparin (1 µg/mL).