Mouse IL-17 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IL-17 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-421-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-421-SP
别名	CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A
全称	Interleukin 17
反应种属	Mouse
应用	Western Blot,CyTOF-ready,Intracellular Staining by Flow Cytometry,Neutralization
目标/特异性	Detects mouse and rat IL-17 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human IL-17A and recombinant mouse (rm) IL-17F is observed and less than 1% cross-reactivity with rmIL-17B, rmIL-17C, rmIL‑17D, and rmIL-17E is observed.
使用方法	Western Blot: 0.1 µg/mL CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND₅₀) is typically 0.05-0.25 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑17.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL17 Promotes Mammary Tumor Progression by Changing the Behavior of Tumor Cells and Eliciting Tumorigenic Neutrophils Recruitment. Authors: Benevides L, da Fonseca D, Donate P, Tiezzi D, De Carvalho D, de Andrade J, Martins G, Silva J Cancer Res, 2015;75(18):3788-99. Species: Mouse Sample Type: In Vivo Application: In vivo Microenvironment-derived IL-1 and IL-17 interact in the control of lung metastasis. Authors: Carmi Y, Rinott G, Dotan S, Elkabets M, Rider P, Voronov E, Apte RN J. Immunol., 2011;186(6):3462-71. Species: Mouse Sample Type: In Vivo Application: In vivo IL-17 and VEGF are necessary for efficient corneal nerve regeneration. Authors: Li Z, Burns AR, Han L, Rumbaut RE, Smith CW Am. J. Pathol., 2011;178(3):1106-16. Species: Mouse Sample Type: In Vivo Application: In vivo Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production. Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ Proc. Natl. Acad. Sci. U.S.A., 2009;106(14):5954-9. Species: Mouse Sample Type: In Vivo Application: In vivo Anti-inflammatory effects of IL-17A on Helicobacter pylori-induced gastritis. Authors: Otani K, Watanabe T, Tanigawa T, Okazaki H, Yamagami H, Watanabe K, Tominaga K, Fujiwara Y, Oshitani N, Arakawa T Biochem. Biophys. Res. Commun., 2009;382(2):252-8. Species: Mouse Sample Type: Whole Tissue Application: IHC Fresh Targeting Tim-1 to overcome resistance to transplantation tolerance mediated by CD8 T17 cells. Authors: Yuan X, Ansari MJ, DAddio F, Paez-Cortez J, Schmitt I, Donnarumma M, Boenisch O, Zhao X, Popoola J, Clarkson MR, Yagita H, Akiba H, Freeman GJ, Iacomini J, Turka LA, Glimcher LH, Sayegh MH Proc. Natl. Acad. Sci. U.S.A., 2009;106(26):10734-9. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Resveratrol inhibits high glucose-induced PI3K/Akt/ERK-dependent interleukin-17 expression in primary mouse cardiac fibroblasts. Authors: Venkatachalam K, Mummidi S, Cortez DM, Prabhu SD, Valente AJ, Chandrasekar B Am. J. Physiol. Heart Circ. Physiol., 2008;294(5):H2078-87. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Tumor-specific Th17-polarized cells eradicate large established melanoma. Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP Blood, 2008;112(2):362-73. Species: Mouse Sample Type: In Vivo Application: In vivo T-bet regulates the fate of Th1 and Th17 lymphocytes in autoimmunity. Authors: Gocke AR, Cravens PD, Ben LH, Hussain RZ, Northrop SC, Racke MK, Lovett-Racke AE J. Immunol., 2007;178(3):1341-8. Species: Mouse Sample Type: Cell Lysates Application: WB IL-17, produced by lymphocytes and neutrophils, is necessary for lipopolysaccharide-induced airway neutrophilia: IL-15 as a possible trigger. Authors: Ferretti S, Bonneau O, Dubois GR, Jones CE, Trifilieff A J. Immunol., 2003;170(4):2106-12. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse IL‑17 Thr22-Ala158 Accession # Q62386
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse, and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, mIL-17 shows 57% and 87% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR⁺CD4^-CD8^-T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kappa B and costimulation of T cell proliferation.
运输条件	Blue Ice
存放说明	4℃
参考文献	Kennedy, J. et al. (1996) J. Interferon Cytokine Res. 16:611. Yao, Z. et al. (1995) J. Immunol. 155:5483. Yao, Z. et al. (1995) Immunity 3:811. Rouvier, E. et al. (1993) J. Immunol. 150:5445.

参考图片

Detection of IL-17 in EL-4 Mouse Cell Line by Flow Cytometry. EL-4 mouse lymphoblast cell line was treated for 16 hours with 50 ng/mL PMA then stained with Goat Anti-Mouse IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-421-NA, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

IL-6 Secretion Induced by IL-17 and Neutralization by Mouse IL-17 Antibody. Recombinant Mouse IL-17 (Catalog # 421-ML) stimulates IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine ELISA Kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Mouse IL-17 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-421-NA). The ND₅₀ is typically
0.05-0.25 µg/mL.