Mouse IL-12 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IL-12 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-419-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-419-SP
别名	CLMF p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35
全称	Interleukin 12
反应种属	Mouse
应用	Western Blot,Neutralization
目标/特异性	Detects mouse IL-12 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing and reducing conditions), this antibody shows less than 10% cross-reactivity with recombinant human IL-12.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IL‑12-induced proliferation in PHA-activated mouse splenocytes. Mattner, F. et al. (1993) Eur. J. Immunol. 23:2202. The Neutralization Dose (ND₅₀) is typically 0.01-0.06 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑12.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3592 (Human); 16159 (Mouse); 84405 (Rat); 403977 (Canine); 493741 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Efficient expression of bioactive murine IL12 as a self-processing P2A polypeptide driven by inflammation-regulated promoters in tumor cell lines. Authors: Lorenzo C, Perez-Chacon G, Garaulet G, Mallorquin Z, Zapata J, Rodriguez A Cancer Gene Ther, 2015;22(11):542-51. Species: Mouse Sample Type: Cell Lysates Application: WB Viral antigen mediated NKp46 activation of NK cells results in tumor rejection via NK-DC crosstalk. Authors: Chinnery, Fay, King, Catherin, Elliott, Tim, Bateman, Andrew R, James, Edward Oncoimmunology, 2012;1(6):874-883. Species: Mouse Sample Type: Whole Cells Application: Neut The C5a receptor impairs IL-12-dependent clearance of Porphyromonas gingivalis and is required for induction of periodontal bone loss. Authors: Liang S, Krauss JL, Domon H, McIntosh ML, Hosur KB, Qu H, Li F, Tzekou A, Lambris JD, Hajishengallis G J. Immunol., 2011;186(2):869-77. Species: Mouse Sample Type: In Vivo Application: In vivo Expression of bioactive single-chain murine IL-12 in transgenic plants. Authors: Liu J, Dolan MC, Reidy M, Cramer CL J. Interferon Cytokine Res., 2009;28(0):381-92. Species: Mouse Sample Type: Tissue Homogenates Application: WB Complement receptor 3 blockade promotes IL-12-Mediated Clearance of Porphyromonas gingivalis and Negates Its Virulence In Vivo. Authors: Hajishengallis G, Shakhatreh MA, Wang M, Liang S J. Immunol., 2007;179(4):2359-67. Species: Mouse Sample Type: In Vivo Application: In vivo Toll-like receptor (TLR)2 and TLR3 synergy and cross-inhibition in murine myeloid dendritic cells. Authors: Vanhoutte F, Paget C, Breuilh L, Fontaine J, Vendeville C, Goriely S, Ryffel B, Faveeuw C, Trottein F Immunol. Lett., 2007;116(1):86-94. Species: Mouse Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑12
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent. Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL‑12 is active on both human and mouse cells, human IL‑12 is not active on murine cells. R&D Systems recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 - G158 peptide and the p35 E159‑A193 peptide can be observed in SDS-PAGE. The biological activity of this cleaved mouse IL‑12 is comparable to that of the intact human IL‑12.
运输条件	Blue Ice
存放说明	4℃
参考文献	Conventional/Classical Dendritic Cells Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines and Receptors in Tfh Cell Differentiation and Function gamma delta T Cells Group 1 ILCs IL-12 Family Inflammatory Mediators Inflammatory/Monocyte-derived Dendritic Cells Isolation, Culture, and Identification of Natural Killer Cells Macrophage Activation Markers Multiple Sclerosis and Myelin Related Molecules Myeloid-derived Suppressor Cells (MDSC) Natural Killer T (NKT) Cells Plasmacytoid Dendritic Cells T Cell Cytokine Signaling Th1 Cells

参考图片

Cell Proliferation Induced by IL‑12 and Neutralization by Mouse IL‑12 Antibody. Recombinant Mouse IL‑12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-419-NA). The ND₅₀ is typically 0.01-0.06 µg/mL.