| 货号 | AF4188-SP |
| 别名 | DBP; DBP/GC; DBP-MAF; DBPVDBG; EC 6.3.1.5; Gc; gc-globulin; Gc-MAF; GRD3; group-specific component (vitamin D binding protein); Group-specific component; hDBP; VDB; VDBG; VDBP; vitamin D-binding alpha-globulin; vitamin D-binding protein | 全称 | Vitamin D Binding Protein |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.5 µg/mL) Simple Western(5 µg/mL) |
| 目标/特异性 | Detects mouse Vitamin D BP in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Vitamin D BP is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2638 (Human); 14473 (Mouse); 24384 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Vitamin D BP Leu17-Ser476 Accession # P21614 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | VDBP (Vitamin D binding protein; also group-specific component and GC-globulin) is a 52‑58 kDa, monomeric glycoprotein member of the ALB/AFP/VDB family of molecules. It is found in blood, urine and CSF, carries Vitamin D and its metabolites, and serves as an actin-scavenging protein. Mature mouse VDBP is 460 amino acids (aa) in length. It contains three albumin-type domains (aa 26‑476) that are accompanied by 14 intrachain disulfide bonds. There are three potential alternative splice forms. One shows a deletion of aa 346‑421, a second shows a 67 aa substitution for aa 345‑421, and a third shows a 34 aa substitution for aa 346‑423. All these variants involve the second and third albumin-like domains. Mature mouse VDBP (aa 17‑476) is 77% and 90% aa identical to human and rat VDBP, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse Vitamin D BP by Western Blot. Western blot shows lysates of mouse liver. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Vitamin D BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4188) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Vitamin D BP at approximately 52-58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Mouse Vitamin D BP by Simple WesternTM. Simple Western lane view shows lysates of mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Vitamin D BP at approximately 58 kDa (as indicated) using 5 µg/mL of Sheep Anti-Mouse Vitamin D BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4188) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. |
