Mouse IL-10 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IL-10 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-417-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-417-SP
别名	CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
全称	Interleukin 10
反应种属	Mouse
应用	Western Blot,Neutralization
目标/特异性	Detects mouse IL-10 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant rat IL‑10 and recombinant cotton rat IL-10 is observed, and less than 5% cross-reactivity with recombinant porcine IL-10, recombinant canine IL-10, recombinant feline IL-10, recombinant equine IL-10, recombinant viral IL-10, recombinant guinea pig IL-10, and recombinant human IL‑10 is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND₅₀) is typically 0.01-0.05 µg/mL in the presence of 2.5 ng/mL Recombinant Mouse IL‑10.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF. Authors: El-Behi M, Ciric B, Dai H Nat. Immunol., 2011;12(6):568-75. Species: Mouse Sample Type: Whole Cells Application: Neut Mutual modulation between interleukin-10 and interleukin-6 induced by Rhodococcus aurantiacus infection in mice. Authors: Yimin, Kohanawa M, Ozaki M, Haga S, Fujikawa K, Zhao S, Kuge Y, Tamaki N Microbes Infect., 2008;10(14):1450-8. Species: Mouse Sample Type: In Vivo Application: In vivo Vascular and inflammatory stresses mediate atherosclerosis via RAGE and its ligands in apoE-/- mice. Authors: Harja E, Bu DX, Hudson BI, Chang JS, Shen X, Hallam K, Kalea AZ, Lu Y, Rosario RH, Oruganti S, Nikolla Z, Belov D, Lalla E, Ramasamy R, Yan SF, Schmidt AM J. Clin. Invest., 2008;118(1):183-94. Species: Mouse Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse IL‑10 Ser19-Ser178 Accession # NP_034678
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL‑20, IL-22, and IL-24. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Mature mouse IL-10 shares 85% amino acid sequence identity with rat and 70%‑77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer. The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL‑10 R beta /IL‑10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3. IL-10 R beta does not bind IL-10 by itself but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL‑22, IL-26, IL-28, and IL-29. IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation. It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Development and Differentiation B Cell Markers Conventional/Classical Dendritic Cells Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines and Receptors in Tfh Cell Differentiation and Function gamma delta T Cells IL-10/Interferon Family Inflammatory Mediators Isolation, Culture, and Identification of Natural Killer Cells Lymphoma Macrophage Activation Markers Molecules Secreted by VSMC Monocyte Markers Multiple Sclerosis and Myelin Related Molecules Myeloid-derived Suppressor Cells (MDSC) Natural Killer T (NKT) Cells Neuroinflammation Plasmacytoid Dendritic Cells Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th1 Cells Th2 Cells Th22 Cells Th9 Cells

参考图片

Detection of Recombinant Mouse IL‑10 by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL‑10 (Catalog #
417-ML), Recombinant Human IL‑10 (Catalog # 217-IL), Recombinant Rat IL‑10 (Catalog # 522-RLB), and Recombinant Human IL‑26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-417-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 Antibody.

Recombinant Mouse IL‑10 (Catalog # 417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-417-NA). The ND₅₀ is typically 0.01‑0.05 µg/mL.