| 货号 | AF-414-SP |
| 别名 | C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin | 全称 | Granulocyte Colony Stimulating Factor |
| 反应种属 | Mouse |
| 应用 | Western Blot,Neutralization |
| 目标/特异性 | Detects mouse G-CSF in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant human G‑CSF is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize G‑CSF-induced proliferation in the NFS‑60 mouse myeloid cell line. Shirafuji, N. et al. (1989) Exp. Hematol. 17:116. The Neutralization Dose (ND50) is typically 0.0025-0.0075 µg/mL in the presence of 0.125 ng/mL Recombinant Mouse G‑CSF. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1440 (Human); 12985 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. G-CSF promotes the proliferation of developing cardiomyocytes in vivo and in derivation from ESCs and iPSCs. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse G-CSF (R&D Systems, Catalog # 414-CS) Val31-Ala208 Accession # P09920 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity. In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by G‑CSF and Neutralization by Mouse G‑CSF Antibody. Recombinant Mouse G‑CSF (Catalog # 414-CS) stimulates proliferation in the NFS‑60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse G‑CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Mouse G‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-414-NA). The ND50 is typically 0.0025-0.0075 µg/mL. |
