Mouse IL-13 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IL-13 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-413-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-413-SP
别名	ALRHMGC116789; BHR1interleukin-13; IL13; IL-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600
全称	Interleukin 13
反应种属	Mouse
应用	Western Blot,Immunocytochemistry,Neutralization
目标/特异性	Detects mouse IL-13 in direct ELISAs and Western blots. In direct ELISAs, approximately 55% cross-reactivity with recombinant rat IL‑13 is observed, and approximately 5% cross-reactivity with recombinant human IL‑13 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.05-0.15 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑13.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Lack of interleukin-13 receptor ?1 delays the loss of dopaminergic neurons during chronic stress Authors: S Mori, S Sugama, W Nguyen, T Michel, MG Sanna, M Sanchez-Al, R Cintron-Co, G Moroncini, Y Kakinuma, P Maher, B Conti J Neuroinflammation, 2017;14(1):88. Species: Mouse Sample Type: Whole Tissue Application: IHC Smoking p66Shc knocked out mice develop respiratory bronchiolitis with fibrosis but not emphysema. Authors: Lunghi B, De Cunto G, Cavarra E, Fineschi S, Bartalesi B, Lungarella G, Lucattelli M PLoS ONE, 2015;10(3):e0119797. Species: Mouse Sample Type: Whole Tissue Application: IHC - Not specified Neutrophils prime a long-lived effector macrophage phenotype that mediates accelerated helminth expulsion. Authors: Chen, Fei, Wu, Wenhui, Millman, Ariel, Craft, Joshua F, Chen, Eunice, Patel, Nirav, Boucher, Jean L, Urban, Joseph F, Kim, Charles, Gause, William Nat Immunol, 2014;15(10):938-46. Species: Mouse Sample Type: Whole Cells Application: Functional Assay Macrophages as IL-25/IL-33-responsive cells play an important role in the induction of type 2 immunity. Authors: Yang Z, Grinchuk V, Urban J, Bohl J, Sun R, Notari L, Yan S, Ramalingam T, Keegan A, Wynn T, Shea-Donohue T, Zhao A , 2013;0(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Diesel exhaust particles induce cysteine oxidation and s-glutathionylation in house dust mite induced murine asthma. Authors: Lee, Gerald B, Brandt, Eric B, Xiao, Chang, Gibson, Aaron M, Le Cras, Timothy, Brown, Lou Ann, Fitzpatrick, Anne M, Khurana Hershey, Gurjit K PLoS ONE, 2013;8(3):e60632. Species: Mouse Sample Type: BALF Application: ELISA Capture Small interfering RNA against transcription factor STAT6 inhibits allergic airway inflammation and hyperreactivity in mice. Authors: Darcan-Nicolaisen Y, Meinicke H, Fels G, Hegend O, Haberland A, Kuhl A, Loddenkemper C, Witzenrath M, Kube S, Henke W, Hamelmann E J. Immunol., 2009;182(12):7501-8. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo. Authors: Chen W, Tabata Y, Gibson AM, Daines MO, Warrier MR, Wills-Karp M, Hershey GK J. Allergy Clin. Immunol., 2008;122(3):625-32. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development The IL-4/IL-13/Stat6 signalling pathway promotes luminal mammary epithelial cell development. Authors: Khaled WT, Read EK, Nicholson SE, Baxter FO, Brennan AJ, Came PJ, Sprigg N, McKenzie AN, Watson CJ Development, 2007;134(15):2739-50. Species: Mouse Sample Type: Whole Cells Application: ICC Modulation of airway remodeling and airway inflammation by peroxisome proliferator-activated receptor gamma in a murine model of toluene diisocyanate-induced asthma. Authors: Lee KS, Park SJ, Kim SR, Min KH, Jin SM, Lee HK, Lee YC J. Immunol., 2006;177(8):5248-57. Species: Mouse Sample Type: BALF Application: WB Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling. Authors: Daines MO, Tabata Y, Walker BA, Chen W, Warrier MR, Basu S, Hershey GK J. Immunol., 2006;176(12):7495-501. Species: Mouse Sample Type: Whole Cells Application: Flow
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse IL-13 Ser26-Phe131 Accession # P20109
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4⁺ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature mouse IL-13 shares 57%, 75%, and 58% amino acid sequence identity with human, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).
运输条件	Blue Ice
存放说明	4℃
参考文献	Wills-Karp, M. (2004) Immunol. Rev. 202:175. Nakajima, H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265. Brown, K.D. et al. (1989) J. Immunol. 142:679. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231. Ruetten, H. and C. Thiemermann (1997) Shock 8:409. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464. Tabata, Y. et al. (2007) J. Immunol. 177:7905. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479. Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.

参考图片

IL‑13 in Mouse Splenocytes. IL‑13 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody. Recombinant Mouse IL‑13 (Catalog # 413-ML) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA). The ND₅₀ is typically 0.05‑0.15 µg/mL.