| 货号 | AF4116-SP |
| 别名 | atrioventricular septal defect 2; AVSD2; CIRRIN; cysteine-rich with EGF-like domain protein 1; cysteine-rich with EGF-like domains 1; DKFZp566D213 | 全称 | Cysteine-rich Protein With EGF-like Domains 1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse CRELD1 in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human CRELD1 is observed and less than 5% cross-reactivity with recombinant mouse CRELD2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 78987 (Human); 171508 (Mouse); 312638 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse CRELD1 Gln30-Glu362 Accession # Q91XD7 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CRELD1 (cysteine-rich with EGF-like domain protein 1) is an integral membrane protein that functions as a cell adhesion molecule. It is synthesized as a 420 amino acid (aa) precursor with an N-terminal signal peptide, a large extracellular domain (ECD) with two EGF-like repeats that flank two consecutive furin-like repeats, two‑transmembrane domains separated by one cytoplasmic residue, and a short extracellular C-terminal domain. CRELD1 null-mouse fails to develop a normal heart and missense mutations in human CRELD1 have also been associated with atrioventricular septal defects. The N-terminal ECD of mouse CRELD1 shares 97% and 92% aa sequence identity with the ECD of rat and human CRELD1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
CRELD1 in Mouse Embryo. CRELD1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Mouse CRELD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4116) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the developing heart and is shown at both low (lower panel) and high magnification (upper panel). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
