| 货号 | AF4105-SP |
| 别名 | BCNS; BCNSFLJ42602; FLJ26746; HPE7; mes; NBCCS; patched (Drosophila) homolog; patched 1; patched homolog (Drosophila); patched homolog 1 (Drosophila); patched homolog 1; protein patched homolog 1; PTC; ptc1; PTCH protein +12b; PTCH protein +4; PTCH protein -10; PTCH; PTCH1; PTCH11 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects mouse Patched 1/PTCH in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Patched 1 is observed, and less than 1% cross-reactivity with rhPatched-2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5727 (Human); 19206 (Mouse); 89830 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Toll-like receptor 3 regulates neural stem cell proliferation by modulating the Sonic Hedgehog pathway. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Patched 1/PTCH Glu108-Asp422 Accession # Q61115 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Patched (Ptch1) is a 170‑175 kDa protein that functions in Hedgehog signaling. It is found on multiple cell types and forms a constitutive, inactivating complex with Smoothened. Upon Hedgehog binding to Patched, Smoothened is released and initiates downstream signaling. Mouse Patched is a 12 transmembrane, 1434 amino acid (aa) glycoprotein that contains two terminal cytoplasmic domains. Only one mRNA species for Ptch1 has been reported in mice. However, two or more splice forms may exist, with variation occurring over the first 150 aa of the N-terminus. Mouse Patched shares 97% and 99% aa sequence identity with human and rat Patched, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Patched 1/PTCH in Neuro‑2A Mouse Cell Line by Flow Cytometry. Neuro‑2A mouse neuroblastoma cell line was stained with Goat Anti-Mouse Patched 1/PTCH (First Extracellular Loop) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4105, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). |
