| 货号 | AF407-SP |
| 别名 | IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF | 全称 | Interleukin 7 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) ELISA Capture (Matched Antibody Pair)(0.2-0.8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Detects mouse IL-7 in ELISAs and Western blots. In sandwich immunoassays, approximately 20% cross-reactivity with recombinant rat IL-7 is observed, and less than 0.1% cross‑reactivity with recombinant human IL-7 is observed. Neutralizes the biological activity of recombinant mouse IL-7. It will not neutralize the biological activity of recombinant human IL-7. |
| 使用方法 | Western Blot: 0.1 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑7-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC). The Neutralization Dose (ND50) is typically 0.2-0.4 µg/mL in the presence of 1.5 ng/mL Recombinant Mouse IL‑7. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3574 (Human); 16196 (Mouse); 25647 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Commensal microflora and interferon-gamma promote steady-state interleukin-7 production in vivo. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse IL-7 Glu26-Ile154 Accession # Q544C8 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | IL-7, previously known as pre-B-cell growth factor and lymphopoietin-1, was originally purified on the basis of its ability to promote the proliferation of precursor B‑cells. It has now been shown that IL-7 can also stimulate the proliferation of thymocytes, T cell progenitors and mature CD4+and CD8+ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). IL-7 was also shown to induce the V(D)J rearrangement of the T cell receptor beta gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumorocidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues. Mouse IL-7 cDNA encodes a precursor protein of 154 amino residues containing a 25 amino acid residue signal peptide. Human IL-7 has approximately 65% amino acid sequence identity with mouse IL-7 and both proteins exhibit cross-species activity. IL-7 bioactivities are mediated by the binding of IL-7 to functional high-affinity receptor complexes. The ligand binding subunit (IL-7 R) of the IL-7 receptor complex has been cloned from human and mouse sources. In addition to the membrane-anchored form of the IL-7 receptor, a human cDNA clone that encodes a soluble form of the IL-7 R has also been isolated. The gamma chain of the IL-2 receptor complex has been shown to be an essential component for IL-7 signal transduction. Both IL-7 R and IL-2 R gamma are members of the hematopoietin receptor superfamily. Cells known to express IL-7 receptors include pre-B cells, T cells and bone marrow cells. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by IL‑7 and Neutralization by Mouse IL‑7 Antibody. Recombinant Mouse IL‑7 (Catalog # 407-ML) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑7 (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF407). The ND50 is typically 0.2-0.4 µg/mL. |
