Mouse IL-6 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IL-6 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-406-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-406-SP
别名	B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A
全称	Interleukin 6
反应种属	Mouse
应用	Western Blot,Immunocytochemistry,Neutralization
目标/特异性	Detects mouse IL-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant rat IL‑6 and recombinant cotton rat IL-6 is observed, 5% cross-reactivity with recombinant human IL-6, recombinant porcine IL-6, recombinant canine IL‑6, and recombinant feline IL‑6 is observed, and less than 1% cross-reactivity with recombinant equine IL-6 is observed.
使用方法	Western Blot: 0.5 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND₅₀) is typically 0.01-0.03 µg/mL in the presence of 0.25 ng/mL Recombinant Mouse IL‑6.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism Authors: C Zhao, W Zhang, Y Zhao, Y Yang, H Luo, G Ji, E Dong, H Deng, S Lin, Y Wei, H Yang Sci Rep, 2016;6(0):19404. Species: Mouse Sample Type: Whole Cells Application: Neut CD8+ T cells produce a dialyzable antigen-specific activator of dendritic cells J Leukoc Biol, 2016;0(0):. Species: Mouse Sample Type: Whole Cells Application: Neut Respiratory tract epithelial cells express retinaldehyde dehydrogenase ALDH1A and enhance IgA production by stimulated B cells in the presence of vitamin A. Authors: Rudraraju R, Jones B, Surman S, Sealy R, Thomas P, Hurwitz J PLoS ONE, 2014;9(1):e86554. Species: Mouse Sample Type: Whole Cells Application: Neut Dysregulation of suppressor of cytokine signaling 3 in keratinocytes causes skin inflammation mediated by interleukin-20 receptor-related cytokines. Authors: Uto-Konomi A, Miyauchi K, Ozaki N PLoS ONE, 2012;7(7):e40343. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Tumor development in murine ulcerative colitis depends on MyD88 signaling of colonic F4/80+CD11b(high)Gr1(low) macrophages. Authors: Schiechl G, Bauer B, Fuss I, Lang SA, Moser C, Ruemmele P, Rose-John S, Neurath MF, Geissler EK, Schlitt HJ, Strober W, Fichtner-Feigl S J. Clin. Invest., 2011;121(5):1692-708. Species: Mouse Sample Type: In Vivo Application: In vivo IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells. Authors: Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS J. Clin. Invest., 2011;121(10):3846-59. Species: Mouse Sample Type: In Vivo Application: In vivo Foxa1 and Foxa2 regulate bile duct development in mice. Authors: Li Z, White P, Tuteja G, Rubins N, Sackett S, Kaestner KH J. Clin. Invest., 2009;119(6):1537-45. Species: Mouse Sample Type: In Vivo Application: In vivo Uropathogenic Escherichia coli-induced inflammation alters mouse urinary bladder contraction via an interleukin-6-activated inducible nitric oxide synthase-related pathway. Authors: Weng TI, Wu HY, Lin PY, Liu SH Infect. Immun., 2009;77(8):3312-9. Species: Mouse Sample Type: In Vivo Application: In vivo The critical role of invading peripheral macrophage-derived interleukin-6 in vincristine-induced mechanical allodynia in mice. Authors: Kiguchi N, Maeda T, Kobayashi Y, Kondo T, Ozaki M, Kishioka S Eur. J. Pharmacol., 2008;592(1):87-92. Species: Mouse Sample Type: In Vivo Application: In vivo Particulate matter exposure induces persistent lung inflammation and endothelial dysfunction. Authors: Tamagawa E, Bai N, Morimoto K, Gray C, Mui T, Yatera K, Zhang X, Xing L, Li Y, Laher I, Sin DD, Man SF, van Eeden SF Am. J. Physiol. Lung Cell Mol. Physiol., 2008;295(1):L79-85. Species: Rabbit Sample Type: Whole Tissue Application: IHC Neuritogenic effects of T cell-derived IL-3 on mouse splenic sympathetic neurons in vivo. Authors: Kannan-Hayashi Y, Okamura K, Hattori S, Kuwamura M, Higuchi E, Terayama H, Moriyama M, Mukamoto M, Okada M, Ohsugi Y, Nakamura Y J. Immunol., 2008;180(6):4227-34. Species: Mouse Sample Type: In Vivo Application: In vivo Mechanisms of interleukin-6 synthesis and release induced by interleukin-1 and cell depolarisation in neurones. Authors: Tsakiri N, Kimber I, Rothwell NJ, Pinteaux E Mol. Cell. Neurosci., 2007;37(1):110-8. Species: Mouse Sample Type: Whole Cells Application: ICC Interleukin-6 as a mechanism for the adverse effects of social stress on acute Theilers virus infection. Authors: Meagher MW, Johnson RR, Young EE, Vichaya EG, Lunt S, Hardin EA, Connor MA, Welsh CJ Brain Behav. Immun., 2007;21(8):1083-95. Species: Mouse Sample Type: In Vivo Application: In vivo Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells. Authors: Kurooka M, Kaneda Y Cancer Res., 2007;67(1):227-36. Species: Mouse Sample Type: Whole Cells Application: Neut High pressure promotes monocyte adhesion to the vascular wall. Authors: Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S Circ. Res., 2007;100(8):1226-33. Species: Mouse Sample Type: Whole Tissue Application: IHC The sex steroid precursor DHEA accelerates cutaneous wound healing via the estrogen receptors. Authors: Mills SJ, Ashworth JJ, Gilliver SC, Hardman MJ, Ashcroft GS J. Invest. Dermatol., 2005;125(5):1053-62. Species: Mouse Sample Type: Tissue Homogenates Application: WB The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice. Authors: Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M Arch. Dermatol. Res., 2001;293(10):508-14. Species: Mouse Sample Type: Whole Cells Application: Neut A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells. Authors: Shih CC, Hu MC, Hu J Blood, 2000;95(6):1957-66. Species: Mouse Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse IL-6 Phe25-Thr211 Accession # P08505
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature mouse IL-6 is 187 amino acids (aa) in length and shares 42% and 85% aa sequence identity with human and rat IL-6, respectively (5). Alternate splicing generates several isoforms with internal deletions (6). Mouse IL-6 is equally active on rat cells (7). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (8). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (9). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (9). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 10).
运输条件	Blue Ice
存放说明	4℃
参考文献	Van Snick, J. (1990) Annu. Rev. Immunol. 8:253. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502. Jones, S.A. (2005) J. Immunol. 175:3468. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227. Van Snick, J. et al. (1988) Eur. J. Immunol. 18:193. Yatsenko, O.P. et al. (2004) Cytokine 28:190. Chiu, C.P. et al. (1988) Proc. Natl. Acad. Sci. 85:7099. Murakami, M. et al. (1993) Science 260:1808. Muller-Newen, G. (2003) Sci. STKE 2003:PE40. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.

参考图片

Detection of Mouse IL‑6 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL‑6 Antibody. Recombinant Mouse IL‑6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA). The ND₅₀ is typically
0.01-0.03 µg/mL.