Mouse IL-1 beta/IL-1F2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IL-1 beta/IL-1F2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-401-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-401-SP
别名	catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
全称	Interleukin 1 beta
反应种属	Mouse
应用	Western Blot,Simple Western,Immunohistochemistry,Immunocytochemistry,Neutralization
目标/特异性	Detects mouse IL-1 beta /IL-1F2 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant rat IL‑1 beta and recombinant porcine IL-1 beta is observed.
使用方法	Western Blot: 0.25 µg/mL Simple Western: 2.5 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically ≤0.25 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 beta /IL‑1F2.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Tetrahydrobiopterin (BH4) deficiency is associated with augmented inflammation and microvascular degeneration in the retina Authors: JC Rivera, B Noueihed, A Madaan, I Lahaie, J Pan, J Belik, S Chemtob J Neuroinflammation, 2017;14(1):181. Species: Mouse Sample Type: Whole Tissue Application: IHC Papilloma-pseudovirus eradicates intestinal tumours and triples the lifespan of Apc(Min/+) mice Authors: Z Zhong, Y Zhai, P Bu, S Shah, L Qiao Nat Commun, 2017;8(0):15004. Species: Mouse Sample Type: Tissue Homogenates Application: WB Toxoplasma gondii GRA7-Targeted ASC and PLD1 Promote Antibacterial Host Defense via PKCï¿½ Authors: HJ Koh, YR Kim, JS Kim, JS Yun, K Jang, CS Yang PLoS Pathog, 2017;13(1):e1006126. Species: Mouse Sample Type: Cell Lysates Application: WB Targeting IRE1 with small molecules counteracts progression of atherosclerosis Authors: O Tufanli, P Telkoparan, D Acosta-Alv, B Kocaturk, UI Onat, SM Hamid, I €imen, P Walter, C Weber, E Erbay Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB Quercetin Inhibits Inflammasome Activation by Interfering with ASC Oligomerization and Prevents Interleukin-1 Mediated Mouse Vasculitis Authors: TP Domiciano, D Wakita, HD Jones, TR Crother, WA Verri, M Arditi, K Shimada Sci Rep, 2017;7(0):41539. Species: Mouse Sample Type: Cell Lysates Application: WB Distinct Kinase-Independent Role of RIPK3 in CD11c(+) Mononuclear Phagocytes in Cytokine-Induced Tissue Repair Authors: K Moriwaki, S Balaji, J Bertin, PJ Gough, FK Chan Cell Rep, 2017;18(10):2441-2451. Species: Mouse Sample Type: Cell Lysates Application: WB The P2X7 Receptor Primes IL-1? and the NLRP3 Inflammasome in Astrocytes Exposed to Mechanical Strain Authors: F Albalawi, W Lu, JM Beckel, JC Lim, SA McCaughey, CH Mitchell Front Cell Neurosci, 2017;11(0):227. Species: Mouse Sample Type: Tissue Homogenates Application: WB MTG16 is a tumor suppressor in colitis-associated carcinoma Authors: EM McDonough, CW Barrett, B Parang, MK Mittal, JJ Smith, AM Bradley, YA Choksi, LA Coburn, SP Short, JJ Thompson, B Zhang, SV Poindexter, MA Fischer, X Chen, J Li, FL Revetta, R Naik, MK Washington, MJ Rosen, SW Hiebert, KT Wilson, CS Williams JCI Insight, 2017;2(16):. Species: Mouse Sample Type: Whole Tissue Application: IHC SR-B1 Is a Silica Receptor that Mediates Canonical Inflammasome Activation Authors: M Tsugita, N Morimoto, M Tashiro, K Kinoshita, M Nakayama Cell Rep, 2017;18(5):1298-1311. Species: Mouse Sample Type: Cell Lysates Application: WB IL-1? transcriptionally activates hepcidin by inducing C/EBP? expression in hepatocytes Authors: Y Kanamori, M Murakami, M Sugiyama, O Hashimoto, T Matsui, M Funaba J. Biol. Chem., 2017;0(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production Authors: H Ahn, E Jeon, JC Kim, SG Kang, SI Yoon, HJ Ko, PH Kim, GS Lee Sci Rep, 2017;7(1):1314. Species: Mouse Sample Type: Cell Lysates Application: WB The intracellular chloride channel proteins CLIC1 and CLIC4 induce IL-1? transcription and activate the NLRP3 inflammasome Authors: R Domingo-Fe, RC Coll, J Kearney, S Breit, LA ONeill J. Biol. Chem., 2017;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB Nucleoside reverse transcriptase inhibitors (NRTIs) induce proinflammatory cytokines in the CNS via Wnt5a signaling Authors: T Wu, J Zhang, M Geng, SJ Tang, W Zhang, J Shu Sci Rep, 2017;7(1):4117. Species: Mouse Sample Type: Tissue Homogenates Application: WB A new approach for ratiometric in vivo calcium imaging of microglia Authors: B Brawek, Y Liang, D Savitska, K Li, N Fomin-Thun, Y Kovalchuk, E Zirdum, J Jakobsson, O Garaschuk Sci Rep, 2017;7(1):6030. The PYHIN Protein p205 Regulates the Inflammasome by Controlling Asc Expression Authors: S Ghosh, C Wallerath, S Covarrubia, V Hornung, S Carpenter, KA Fitzgerald J. Immunol., 2017;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB Cutting Edge: Distinct Regulatory Mechanisms Control Proinflammatory Cytokines IL-18 and IL-1? Authors: Q Zhu, TD Kanneganti J. Immunol., 2017;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB C13orf31 (FAMIN) is a central regulator of immunometabolic function Nat Immunol, 2016;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB Lymphatic vessels regulate immune microenvironments in human and murine melanoma J Clin Invest, 2016;0(0):. Species: Human Sample Type: Tissue Homogenates Application: WB Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS Authors: Yong Hwan Park Nat Immunol, 2016;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB Enzymatically inactive procaspase-1 stabilizes the ASC-pyroptosome and supports pyroptosome spreading during cell division J Biol Chem, 2016;0(0):. Species: Mouse Sample Type: Cell Culture Supernates Application: Bead-based Immunoassay The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation Authors: X Wang, DK Shaw, OS Sakhon, GA Snyder, EJ Sundberg, L Santambrog, FS Sutterwala, JS Dumler, KA Shirey, DJ Perkins, K Richard, AC Chagas, E Calvo, J Kopecký, M Kotsyfakis, JH Pedra Infect Immun, 2016;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB Functional and Evolutionary Analyses Identify Proteolysis as a General Mechanism for NLRP1 Inflammasome Activation PLoS Pathog, 2016;12(12):e1006052. Species: Human Sample Type: Cell Lysates Application: WB CARD9 negatively regulates NLRP3-induced IL-1? production on Salmonella infection of macrophages Nat Commun, 2016;7(0):12874. Species: Mouse Sample Type: Cell Lysates Application: WB TNF?-stimulated gene-6 (TSG6) activates macrophage phenotype transition to prevent inflammatory lung injury Proc. Natl. Acad. Sci. U.S.A., 2016;113(50):E8151-E8158. Species: Mouse Sample Type: Cell Lysates Application: WB Histone deacetylase inhibitors mediate DNA damage repair in ameliorating hemorrhagic cystitis Sci Rep, 2016;6(0):39257. Species: Mouse Sample Type: Tissue Homogenates Application: WB Activation of NLRP3 inflammasomes in mouse hepatic stellate cells during Schistosoma J. infection Oncotarget, 2016;7(26):39316-39331. Species: Mouse Sample Type: Whole Tissue Application: IF Identification of QS-21 as an Inflammasome-activating Molecular Component of Saponin Adjuvants. Authors: Marty-Roix R, Vladimer G, Pouliot K, Weng D, Buglione-Corbett R, West K, Macmicking J, Chee J, Wang S, Lu S, Lien E J Biol Chem, 2016;291(3):1123-36. Species: Mouse Sample Type: Cell Lysates Application: WB CD8+ T cells produce a dialyzable antigen-specific activator of dendritic cells J Leukoc Biol, 2016;0(0):. Species: Mouse Sample Type: Whole Cells Application: Neut HIF-1? Is an Essential Mediator of IFN-?-Dependent Immunity to Mycobacterium tuberculosis J Immunol, 2016;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB IL-10 engages macrophages to shift Th17 cytokine dependency and pathogenicity during T-cell-mediated colitis. Authors: Li B, Gurung P, Malireddi R, Vogel P, Kanneganti T, Geiger T Nat Commun, 2015;6(0):6131. Species: Mouse Sample Type: Tissue Homogenates Application: WB The cyclopentenone prostaglandin 15d-PGJ2 inhibits the NLRP1 and NLRP3 inflammasomes. Authors: Maier N, Leppla S, Moayeri M J Immunol, 2015;194(6):2776-85. Species: Mouse Sample Type: Cell Lysates Application: WB Caspase-1-independent IL-1 release mediates blister formation in autoantibody-induced tissue injury through modulation of endothelial adhesion molecules. Authors: Sadeghi H, Lockmann A, Hund A, Samavedam U, Pipi E, Vafia K, Hauenschild E, Kalies K, Pas H, Jonkman M, Iwata H, Recke A, Schon M, Zillikens D, Schmidt E, Ludwig R J Immunol, 2015;194(8):3656-63. RIPK3 promotes cell death and NLRP3 inflammasome activation in the absence of MLKL. Authors: Lawlor K, Khan N, Mildenhall A, Gerlic M, Croker B, DCruz A, Hall C, Kaur Spall S, Anderton H, Masters S, Rashidi M, Wicks I, Alexander W, Mitsuuchi Y, Benetatos C, Condon S, Wong W, Silke J, Vaux D, Vince J Nat Commun, 2015;6(0):6282. Species: Mouse Sample Type: Cell Lysates Application: WB Deficient NLRP3 and AIM2 Inflammasome Function in Autoimmune NZB Mice. Authors: Sester D, Sagulenko V, Thygesen S, Cridland J, Loi Y, Cridland S, Masters S, Genske U, Hornung V, Andoniou C, Sweet M, Degli-Esposti M, Schroder K, Stacey K J Immunol, 2015;195(3):1233-41. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida. Authors: Meunier E, Wallet P, Dreier R, Costanzo S, Anton L, Ruhl S, Dussurgey S, Dick M, Kistner A, Rigard M, Degrandi D, Pfeffer K, Yamamoto M, Henry T, Broz P , 2015;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB Local immunosuppressive microenvironment enhances migration of melanoma cells to lungs in DJ-1 knockout mice. Authors: Chien C, Lee M, Liou H, Liou H, Fu W PLoS ONE, 2015;10(2):e0115827. Species: Mouse Sample Type: In Vivo Application: In vivo AIM2 and NLRC4 inflammasomes contribute with ASC to acute brain injury independently of NLRP3. Authors: Denes A, Coutts G, Lenart N, Cruickshank S, Pelegrin P, Skinner J, Rothwell N, Allan S, Brough D Proc Natl Acad Sci U S A, 2015;112(13):4050-5. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Retinal neurons curb inflammation and enhance revascularization in ischemic retinopathies via proteinase-activated receptor-2. Authors: Sitaras N, Rivera J, Noueihed B, Bien-Aime M, Zaniolo K, Omri S, Hamel D, Zhu T, Hardy P, Sapieha P, Joyal J, Chemtob S Am J Pathol, 2015;185(2):581-95. Species: Mouse Sample Type: Tissue Homogenates Application: WB Methylsulfonylmethane inhibits NLRP3 inflammasome activation. Authors: Ahn H, Kim J, Lee M, Kim Y, Cho Y, Lee G Cytokine, 2015;71(2):223-31. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Small heterodimer partner interacts with NLRP3 and negatively regulates activation of the NLRP3 inflammasome. Authors: Yang C, Kim J, Kim T, Lee P, Kim S, Lee H, Shin D, Nguyen L, Lee M, Jin H, Kim K, Lee C, Kim M, Park S, Kim J, Choi H, Jo E Nat Commun, 2015;6(0):6115. Species: Mouse Sample Type: Cell Lysates Application: WB RNA and beta-hemolysin of group B Streptococcus induce interleukin-1beta (IL-1beta) by activating NLRP3 inflammasomes in mouse macrophages. Authors: Gupta R, Ghosh S, Monks B, DeOliveira R, Tzeng T, Kalantari P, Nandy A, Bhattacharjee B, Chan J, Ferreira F, Rathinam V, Sharma S, Lien E, Silverman N, Fitzgerald K, Firon A, Trieu-Cuot P, Henneke P, Golenbock D J Biol Chem, 2014;289(20):13701-5. Species: Mouse Sample Type: Cell Culture Supernates Application: WB 3,4-methylenedioxy-beta-nitrostyrene inhibits NLRP3 inflammasome activation by blocking assembly of the inflammasome. Authors: He Y, Varadarajan S, Munoz-Planillo R, Burberry A, Nakamura Y, Nunez G J Biol Chem, 2014;289(2):1142-50. Species: Mouse Sample Type: Cell Lysates Application: WB Systemic autophagy insufficiency compromises adaptation to metabolic stress and facilitates progression from obesity to diabetes. Authors: Lim, Yu-Mi, Lim, Hyejin, Hur, Kyu Yeon, Quan, Wenying, Lee, Hae-Youn, Cheon, Hwanju, Ryu, Dongryeo, Koo, Seung-Ho, Kim, Hong Lim, Kim, Jin, Komatsu, Masaaki, Lee, Myung-Sh Nat Commun, 2014;5(0):4934. Species: Mouse Sample Type: Tissue Homogenates Application: WB Arsenic trioxide and other arsenical compounds inhibit the NLRP1, NLRP3, and NAIP5/NLRC4 inflammasomes. Authors: Maier N, Crown D, Liu J, Leppla S, Moayeri M J Immunol, 2014;192(2):763-70. Species: Human Sample Type: Cell Lysates Application: WB Caspase-8 modulates dectin-1 and complement receptor 3-driven IL-1beta production in response to beta-glucans and the fungal pathogen, Candida albicans. Authors: Ganesan S, Rathinam V, Bossaller L, Army K, Kaiser W, Mocarski E, Dillon C, Green D, Mayadas T, Levitz S, Hise A, Silverman N, Fitzgerald K J Immunol, 2014;193(5):2519-30. Species: Mouse Sample Type: Cell Lysates Application: WB The frustrated host response to Legionella pneumophila is bypassed by MyD88-dependent translation of pro-inflammatory cytokines. Authors: Asrat S, Dugan A, Isberg R PLoS Pathog, 2014;10(7):e1004229. Species: Mouse Sample Type: Cell Lysates Application: WB Critical role of aquaporins in interleukin 1beta (IL-1beta)-induced inflammation. Authors: Rabolli V, Wallemme L, Lo Re S, Uwambayinema F, Palmai-Pallag M, Thomassen L, Tyteca D, Octave J, Marbaix E, Lison D, Devuyst O, Huaux F J Biol Chem, 2014;289(20):13937-47. Species: Mouse Sample Type: Cell Lysates Application: WB Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases. Authors: Meunier E, Dick M, Dreier R, Schurmann N, Kenzelmann Broz D, Warming S, Roose-Girma M, Bumann D, Kayagaki N, Takeda K, Yamamoto M, Broz P Nature, 2014;509(7500):366-70. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Severe acute respiratory syndrome coronavirus envelope protein ion channel activity promotes virus fitness and pathogenesis. Authors: Nieto-Torres J, DeDiego M, Verdia-Baguena C, Jimenez-Guardeno J, Regla-Nava J, Fernandez-Delgado R, Castano-Rodriguez C, Alcaraz A, Torres J, Aguilella V, Enjuanes L PLoS Pathog, 2014;10(5):e1004077. Species: Mouse Sample Type: Tissue Homogenates Application: WB A critical role for human caspase-4 in endotoxin sensitivity. Authors: Kajiwara Y, Schiff T, Voloudakis G, Gama Sosa M, Elder G, Bozdagi O, Buxbaum J J Immunol, 2014;193(1):335-43. Species: Mouse Sample Type: Cell Lysates Application: WB Signaling pathways in murine dendritic cells that regulate the response to vesicular stomatitis virus vectors that express flagellin. Authors: Smedberg J, Westcott M, Ahmed M, Lyles D J Virol, 2014;88(2):777-85. Species: Mouse Sample Type: Cell Lysates Application: WB Critical role for IL-1beta in DNA damage-induced mucositis. Authors: Kanarek N, Grivennikov S, Leshets M, Lasry A, Alkalay I, Horwitz E, Shaul Y, Stachler M, Voronov E, Apte R, Pagano M, Pikarsky E, Karin M, Ghosh S, Ben-Neriah Y Proc Natl Acad Sci U S A, 2014;111(6):E702-11. Species: Mouse Sample Type: Whole Tissue Application: IHC Nuclear factor E2-related factor-2 (Nrf2) is required for NLRP3 and AIM2 inflammasome activation. Authors: Zhao C, Gillette D, Li X, Zhang Z, Wen H J Biol Chem, 2014;289(24):17020-9. Species: Mouse Sample Type: Cell Lysates Application: WB Activation of NLRP3 inflammasome by crystalline structures via cell surface contact. Authors: Hari A, Zhang Y, Tu Z, Detampel P, Stenner M, Ganguly A, Shi Y Sci Rep, 2014;4(0):7281. Species: Mouse Sample Type: Cell Lysates Application: WB Endothelial NLRP3 inflammasome activation and enhanced neointima formation in mice by adipokine visfatin. Authors: Xia M, Boini K, Abais J, Xu M, Zhang Y, Li P Am J Pathol, 2014;184(5):1617-28. Species: Mouse Sample Type: Whole Tissue Application: IHC Gli1 deletion prevents Helicobacter-induced gastric metaplasia and expansion of myeloid cell subsets. Authors: El-Zaatari M, Kao J, Tessier A, Bai L, Hayes M, Fontaine C, Eaton K, Merchant J PLoS ONE, 2013;8(3):e58935. Species: Mouse Sample Type: Whole Tissue Application: IHC Fluorescence Activation of microglia induces symptoms of Parkinson's disease in wild-type, but not in IL-1 knockout mice. Authors: Tanaka S, Ishii A, Ohtaki H, Shioda S, Yoshida T, Numazawa S J Neuroinflammation, 2013;10(0):143. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen The role of IL-1beta in the early tumor cell-induced angiogenic response. Authors: Carmi Y, Dotan S, Rider P, Kaplanov I, White M, Baron R, Abutbul S, Huszar M, Dinarello C, Apte R, Voronov E J Immunol, 2013;190(7):3500-9. Species: Mouse Sample Type: In Vivo Application: In vivo Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of pro-inflammatory cytokines via the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and c-Jun N-terminal kinase (JNK) signaling pathways. Authors: Li B, Shi Y, Shu J, Gao J, Wu P, Tang S J Biol Chem, 2013;288(19):13610-9. Species: Mouse Sample Type: Tissue Homogenates Application: WB Microtubule-driven spatial arrangement of mitochondria promotes activation of the NLRP3 inflammasome. Authors: Misawa T, Takahama M, Kozaki T, Lee H, Zou J, Saitoh T, Akira S Nat Immunol, 2013;14(5):454-60. Species: Mouse Sample Type: Peritoneal Fluid Application: WB Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus. Authors: Gessner MA, Werner JL, Lilly LM, Nelson MP, Metz AE, Dunaway CW, Chan YR, Ouyang W, Brown GD, Weaver CT, Steele C Infect. Immun., 2012;80(1):410-7. Species: Mouse Sample Type: Whole Cells Application: Neut Caspase-11 increases susceptibility to Salmonella infection in the absence of caspase-1. Authors: Broz P, Ruby T, Belhocine K, Bouley D, Kayagaki N, Dixit V, Monack D Nature, 2012;490(7419):288-91. Species: Mouse Sample Type: Cell Culture Supernates Application: WB The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP. Authors: Lee G, Subramanian N, Kim A, Aksentijevich I, Goldbach-Mansky R, Sacks D, Germain R, Kastner D, Chae J Nature, 2012;492(7427):123-7. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Microenvironment-derived IL-1 and IL-17 interact in the control of lung metastasis. Authors: Carmi Y, Rinott G, Dotan S, Elkabets M, Rider P, Voronov E, Apte RN J. Immunol., 2011;186(6):3462-71. Species: Mouse Sample Type: In Vivo Application: In vivo Coordinated Host Responses during Pyroptosis: Caspase-1-Dependent Lysosome Exocytosis and Inflammatory Cytokine Maturation. Authors: Bergsbaken T, Fink SL, den Hartigh AB, Loomis WP, Cookson BT J. Immunol., 2011;187(5):2748-54. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Toll-like receptor 2 contributes to chemokine gene expression and macrophage infiltration in the dorsal root ganglia after peripheral nerve injury. Authors: Kim D, You B, Lim H, Lee SJ Mol Pain, 2011;7(0):74. Species: Mouse Sample Type: Whole Cells Application: ICC IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells. Authors: Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS J. Clin. Invest., 2011;121(10):3846-59. Species: Mouse Sample Type: In Vivo Application: In vivo IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation. Authors: Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN J. Immunol., 2011;187(9):4835-43. Species: Mouse
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse IL-1 beta /IL-1F2 Val118-Ser269 Accession # NP_032387
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65-78% identity with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Biomarkers Conventional/Classical Dendritic Cells Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis IL-1 Family Infectious Diseases Inflammatory Mediators Isolation, Culture, and Identification of Natural Killer Cells LTi Cells Macrophage Activation Markers Multiple Sclerosis and Myelin Related Molecules Myeloid-derived Suppressor Cells (MDSC) NCR-ILC3 Neuroinflammation NOD-like Receptors and the Inflammasome Platelets Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th17 Cells Th9 Cells

参考图片

Detection of Human and Mouse
IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-)ortreated (+) with 200 nM PMA for 24 hoursand 10 μg/mL LPS for 4 hours andRAW 264.7 mouse monocyte/macrophagecell line untreated (-)or treated (+) with10 μg/mL LPS for 24 hours. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑1 beta /IL‑1F2 in MCF‑7 Human Cell Line. IL‑1 beta /IL‑1F2 was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

IL‑1 beta /IL‑1F2 in Mouse Thymus. IL‑1 beta /IL‑1F2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse IL‑1 beta /IL‑1F2 by Simple Western^TM. Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours, loaded at 0.5 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 40 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse
IL-1 beta /IL-1F2 Antibody. Recombinant Mouse
IL‑1 beta /IL‑1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA). The ND₅₀ is typically ≤0.25 µg/mL.