Mouse Laminin alpha 4 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse Laminin alpha 4 Affinity Purified Polyclonal Ab (25 UG)

货号： AF3837-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3837-SP
别名	DKFZp686D23145; LAMA3LAMA4*-1; LAMA4; laminin alpha 4 chain; laminin subunit alpha-4; laminin, alpha 4; Laminin-14 subunit alpha; Laminin-8 subunit alpha; Laminin-9 subunit alpha
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse Laminin alpha 4 in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3910 (Human); 16775 (Mouse); 309816 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Prenatal muscle development in a mouse model for the secondary dystroglycanopathies. Authors: Kim J, Hopkinson M, Kavishwar M, Fernandez-Fuente M, Brown S Skelet Muscle, 2015;6(0):3. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity. Authors: Yao Y, Chen Z, Norris E, Strickland S Nat Commun, 2014;5(0):3413. Species: Mouse Sample Type: Whole Tissue Application: IHC - Not specified Transforming growth factor ÃŽÂ²-1 stimulates profibrotic epithelial signaling to activate pericyte-myofibroblast transition in obstructive kidney fibrosis. Am J Pathol, 2013;182(1):118-31. Species: Mouse Sample Type: tissue Application: IHC - Not specified Laminin-411 Is a Vascular Ligand for MCAM and Facilitates TH17 Cell Entry into the CNS. Authors: Flanagan K, Fitzgerald K, Baker J PLoS ONE, 2012;7(7):e40443. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Fukutin-related protein alters the deposition of laminin in the eye and brain. Authors: Ackroyd MR, Whitmore C, Prior S, Kaluarachchi M, Nikolic M, Mayer U, Muntoni F, Brown SC J. Neurosci., 2011;31(36):12927-35. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse Laminin alpha 4 Gln826-Ala1816 Accession # P97927
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Laminins are heterotrimeric glycoproteins and are major components of the basement membrane. Each laminin is comprised of a single alpha, beta, and gamma chain that heterotrimerize via their coiled-coil domains to form a large cruciform-shaped molecule. Mature Laminin alpha 4 is a subunit of Laminin 411, Laminin 421, and Laminin 423. Mature Laminin alpha 4 is a 1792 amino acid (aa) residue protein that has a Laminin N-terminal domain, four Laminin EGF-like domains, the coiled-coil domain, and five C‑terminal tandem Laminin G-like domains. The G-like domains contain binding sites for integrin, heparin, and dystroglycan. Within the region used as immunogen, mouse Laminin alpha 4 shows 91% and 97% aa sequence identity with human and rat Laminin alpha 4, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Actin Stress Fibers and Focal Adhesions During EMT Extracellular Matrix and Related Molecules Extracellular Matrix Molecules

参考图片

Laminin alpha 4 in Mouse Embryo. Laminin alpha 4 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Laminin alpha 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3837) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to membranes in blood vessels in CNS. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.