| 货号 | AF3730-SP |
| 别名 | developmental pluripotency associated 4; developmental pluripotency-associated protein 4; FLJ10713,2410091M23Rik | 全称 | Developmental Pluripotency Associated 4 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse DPPA4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human DPPA4 and recombinant mouse DPPA5 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 55211 (Human); 73693 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Identification of DPPA4 and DPPA2 as a novel family of pluripotency-related oncogenes. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse DPPA4 Asn125-Gly296 Accession # Q8CCG4 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | DPPA4 is a 33 kDa molecule that is expressed specifically in the early embryo, uncommitted cells of the germ line, and embryonic stem cells and is used as a marker of pluripotent cells. Mouse DPPA4 is 296 amino acids in length and contains an SAP (SAF-A/B, ACINUS, PIAS) putative DNA-binding domain. Two potential isoforms exist with deletions of amino acids 104‑120 and 69‑120, respectively. In the region used as an immunogen, mouse DPPA4 shows 57% amino acid sequence identity with human and 87% identity with rat DPPA4 proteins. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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DPPA4 in D3 Mouse Cell Line. DPPA4 was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Mouse DPPA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3730) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
