| 货号 | AF3688-SP |
| 别名 | D6Bwg0781e; Img; LIG1; LIG-1 | 全称 | Leucine-rich Repeats and Immunoglobulin-like Domains 1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(2 µg/mL) Flow Cytometry(0.25 µg/106cells) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse LRIG1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) LRIG1 is observed and less than 5% cross-reactivity with rhLRIG3 is observed. |
| 使用方法 | Western Blot: 2 µg/mL Flow Cytometry: 0.25 µg/106cells Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 26018 (Human); 16206 (Mouse); 312574 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. GPR39 marks specific cells within the sebaceous gland and contributes to skin wound healing. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse LRIG1 Ala37-Thr794 Accession # P70193 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | LRIG-1 (leucine-rich repeats and Ig-like domains-1; alsoLIG-1) is an approximately 130-145 kDa glycoprotein that belongs to the LRIGgene family. It is widely expressed, andappears on the surface of prostatic epithelium, endothelial cells, vascular andvisceral smooth muscle, mammary epithelium, cardiac muscle, keratinocytes andneurons. LRIG-1 is believed to negativelyregulate the ErbB family of receptors. In particular, and in a ligand-independent manner, LRIG-1 complexes withall four ErbBs, promoting their ubiquitination and decreasing theirnumber. Alternatively, LRIG-1 is suggestedto bind to the ErbBs, preventing their dimerization and signaltransduction. Mature mouse LRIG-1 is a1057 amino acid (aa) type I transmembrane protein (SwissProt #:P70193). It contains a large 762 amino acid (aa)extracellular domain (ECD) (aa 35-795) plus a 274 aa cytoplasmic region. The ECD contains 17 LRRs (aa’s 35-491) andthree C2-type Ig-like domains (aa’s 497-781). These two domain types are each sufficient for EGFR binding. There one potential alternative splice formthat a deletion of aa 875-923. The LRIG-1ECD undergoes proteolysis, generating 90-105 and 60-70 kDa solublefragments. Over aa 37-794, human LRIG-1 shares97% and 90% aa sequence identity with rat and human LRIG-1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse LRIG1 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line untreated (-), treated (+) or mock treated (m) with 30 pmol mouse LRIG1 or mouse ERK1 siRNA. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for LRIG1 at approximately 140 kDa and 70 kDa (as indicated, upper panel). For additional reference ERK1 was detected using Rabbit Anti-Human/Mouse/Rat ERK1 Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF1575) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of LRIG1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse LRIG1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3688, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). | |
LRIG1 in bEnd.3 Mouse Cell Line. LRIG1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
