Mouse GLI-2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse GLI-2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF3635-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3635-SP
别名	GLI family zinc finger 2; GLI-Kruppel family member GLI2; glioma-associated oncogene family zinc finger 2; HPE9tax helper protein 1; oncogene GLI2; tax helper protein 2; Tax helper protein; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; THP; THP1; THP2; zinc finger protein GLI2
全称	GLI-Kruppel family member GLI2
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse GLI-2 in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2736 (Human); 14633 (Mouse); 304729 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Pharmacological GLI2 inhibition prevents myofibroblast cell-cycle progression and reduces kidney fibrosis. Authors: Kramann R, Fleig S, Schneider R, Fabian S, DiRocco D, Maarouf O, Wongboonsin J, Ikeda Y, Heckl D, Chang S, Rennke H, Waikar S, Humphreys B J Clin Invest, 2015;125(8):2935-51. Species: Mouse Sample Type: Tissue Homogenates Application: WB Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators. Authors: Liu Y, Tsai I, Morleo M, Oh E, Leitch C, Massa F, Lee B, Parker D, Finley D, Zaghloul N, Franco B, Katsanis N J Clin Invest, 2014;124(5):2059-70. Species: Mouse Sample Type: Cell Lysates Application: WB The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins. Authors: Humke EW, Dorn KV, Milenkovic L, Scott MP, Rohatgi R Genes Dev., 2010;24(7):670-82. Species: Mouse Sample Type: Cell Lysates Application: IP
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse GLI-2 Met1-Glu416 Accession # NP_001074594
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Mouse GLI-2 is a 185 kDa biopotential transcription regulator of the hedgehog signaling pathway. It contains 5 conserved tandem C2H2 zinc finger domains that are flanked by a repression domain at the N-terminal region and an activation domain in the C-terminal region. At least two additional isoforms that have amino acid substitutions/truncations in the region corresponding to amino acid residues 192‑350 of isoform 1 have been described. Transcriptional activity of GLI-2 is regulated by proteolytic processing and degradation. Within the region used as immunogen, mouse GLI-2 shares 91% and 97% amino acid sequence homology with that of human and rat GLI-2, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Biomarkers Cancer Stem Cell Markers Cancer Stem Cell Transcription Factors Hedgehog Family ICC / IHC Images: GLI-2 Neural Stem Cell Transcription Factors Transcription Factors in the Akt Pathway

参考图片

GLI‑2 in Mouse Embryo. GLI‑2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse GLI‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3635) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing muscle. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.