Mouse Collagen II Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse Collagen II Affinity Purified Polyclonal Ab (25 UG)

货号： AF3615-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3615-SP
别名	Alpha-1 type II collagen; ANFH; AOM; cartilage collagen; Chondrocalcin; COL11A3; COL2A1; Collagen 2; collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen type 2; collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital); collagen, type II, alpha 1; Collagen-2; MGC131516; SEDC
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects mouse Collagen II in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1280 (Human); 12824 (Mouse); 25412 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Bone marrow-derived multipotent stromal cells attenuate inflammation in obliterative airway disease in mouse tracheal allografts. Authors: Casey A, Dirks F, Liang O, Harrach H, Schuette-Nuetgen K, Leeman K, Kim C, Gerard C, Subramaniam M Stem Cells Int, 2014;2014(0):468927. Species: Mouse Sample Type: whole cells Application: IHC Paraffin-embedded Mesenchymal stromal cells improve salivary function and reduce lymphocytic infiltrates in mice with Sjogren's-like disease. Authors: Khalili S, Liu Y, Kornete M, Roescher N, Kodama S, Peterson A, Piccirillo CA, Tran SD PLoS ONE, 2012;7(6):e38615. Species: Mouse Sample Type: Whole Cells Application: ICC Nkx3.2 promotes primary chondrogenic differentiation by upregulating Col2a1 transcription. Authors: Kawato Y, Hirao M, Ebina K, Shi K, Hashimoto J, Honjo Y, Yoshikawa H, Myoui A PLoS ONE, 2012;7(4):e34703. Species: Mouse Sample Type: Cell Lysates Application: WB Chondroitin sulfate N-acetylgalactosaminyltransferase 1 is necessary for normal endochondral ossification and aggrecan metabolism. Authors: Sato T, Kudo T, Ikehara Y, Ogawa H, Hirano T, Kiyohara K, Hagiwara K, Togayachi A, Ema M, Takahashi S, Kimata K, Watanabe H, Narimatsu H J. Biol. Chem., 2011;286(7):5803-12. Species: Mouse Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse sternum-derived Collagen II
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Mouse Type II Collagen is a 100 kDa fibrillar collagen found in cartilage, vitreous body and vertebral disk. It is synthesized as a 1459 amino acid (aa) preproprecursor that contains an N-terminal and a C-terminal proregion. Intracellularly, type II Collagen homotrimerizes into a fibril and is then secreted. The proregions are then cleaved by procollagenases and the processed homotrimer covalently crosslinks to other type II fibrils. Mature mouse Collagen II shares 95% and 98% aa sequence identity with human and rat Collagen II, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Chondrogenesis Markers Coagulation Extracellular Matrix and Related Molecules Extracellular Matrix Molecules ICC / IHC Images: Collagen II Interactive Pathway: Articular Cartilage Extracellular Matrix Osteogenesis Markers Osteoprogenitor Markers

参考图片

Collagen II in Mouse Chondrocytes. Collagen II was detected in immersion fixed mouse mesenchymal stem cell-differentiated chondrocytes using 10 µg/mL Sheep Anti-Mouse Collagen II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3615) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.