| 货号 | AF3565-SP |
| 别名 | Brain trypsinogen; EC 3.4.21; EC 3.4.21.4; Mesotrypsin; mesotrypsinogen; MTG; pancreatic trypsinogen III; protease, serine, 3 (mesotrypsin); protease, serine, 3; protease, serine, 4 (trypsin 4, brain); PRSS3; PRSS4; Serine protease 3; Serine protease 4; T9; TRY3MTG; TRY4mesotrypsin; trypsin 3; Trypsin 4; Trypsin III; Trypsin IV; trypsin-3; Trypsinogen 15; trypsinogen 4; trypsinogen 5; trypsinogen IV |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects mouse Trypsin 3 in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Trypsin 1, rhTrypsin 2, and rhTrypsin 3 is shown. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5646 (Human); 22073 (Mouse); 362347 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Trypsin 3 Phe16-Asn246 Accession # NP_035775 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Mouse Trypsin 3, encoded by the PRSS3 gene, is also known as mesotrypsin. It consists of a signal peptide (residues 1-15), a pro region (residues 16-23), and a mature chain (residues 24-246) (1). The purified recombinant mouse Trypsin 3 corresponds to the pro form, which can be activated by enterokinase. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Trypsin 3/PRSS3 in Mouse Pancreas. Trypsin 3/PRSS3 was detected in perfusion fixed frozen sections of mouse pancreas using 5 µg/mL Goat Anti-Mouse Trypsin 3/PRSS3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3565) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. | |
Trypsin 3/PRSS3 in Mouse Pancreas. Trypsin 3/PRSS3 was detected in perfusion fixed frozen sections of mouse pancreas using Mouse Trypsin 3/PRSS3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3565) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
