货号 | AF3300-SP |
别名 | EC 3.4.24.63; endopeptidase-2; MEP1B; meprin A subunit beta; meprin A, beta; Meprin B; N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta; PABA peptide hydrolase; PPH beta |
反应种属 | Mouse |
应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL) |
目标/特异性 | Detects mouse Meprin beta Subunit/MEP1B in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human MEP1B is observed. |
使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 4225 (Human); 17288 (Mouse) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Meprin ? contributes to collagen deposition in lung fibrosis | |
纯化方式 | Antigen Affinity-purified |
免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Meprin beta Subunit/MEP1B Leu21-Ser594 (Thr75Ile, Ile432Val) Accession # Q61847 |
生物活性 | Mouse |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
背景 | Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide-linked homo- or heterooligomers, which are also referred to as meprin A (composed of alpha subunits with or without beta subunits) and meprin B (composed of beta subunits only) (3). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 704 amino acid sequence of mouse meprin beta subunit precursor consists of a signal peptide (residues 1-20), a pro region (residues 21-62), and a mature chain (residues 63-704) containing following domains, catalytic (residues 63-260), MAM (residues 261-430), MATH (residues 431-586), EGF-like (residues 607-647), transmembrane (residues 655-678), and cytoplasmic (residues 679-704). The pro enzyme terminating at residue 594 was expressed and the secreted protein purified from conditioned medium. The amino acid sequence has Ile and Val at position 75 and 432 instead of Thr and Ile, respectively. After trypsin treatment, the activated enzyme cleaved a flurogenic peptide, which contains Asp and Glu, the preferred residues found in the P1’ and P1 sites (3). |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Mouse Meprin beta Subunit/MEP1B by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse Meprin beta Subunit/MEP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3300) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Meprin beta Subunit/MEP1B at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5. | |
Meprin beta Subunit/MEP1B in Mouse Intestine. Meprin beta Subunit/MEP1B was detected in perfusion fixed frozen sections of mouse intestine using 1 µg/mL Goat Anti-Mouse Meprin beta Subunit/MEP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3300) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the brush border of intestinal villi.View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |