| 货号 | AF3299-SP |
| 别名 | 6-O-methylguanine-DNA methyltransferase; EC 2.1.1.63; methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA methyltransferase; O6-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase | 全称 | O-6 Methylguanine-DNA Methyltransferase |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse MGMT in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4255 (Human); 17314 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse MGMT Met1-Asn211 Accession # P26187 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The O-6-methylguanine-DNA-methyltransferase (MGMT) is involved in the direct repair of DNA alkylated at the O6 position of guanine. MGMT transfers the alkyl group to its own cysteine thereby inactivating the MGMT molecule. Therefore, the number of MGMT molecules correlates directly with the cell’s ability to withstand DNA alkylation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse MGMT by Western Blot. Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse MGMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3299) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MGMT at approximately 25 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. |
