Mouse Podoplanin Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse Podoplanin Affinity Purified Polyclonal Ab (25 UG)

货号： AF3244-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3244-SP
别名	36-KD; Aggrus; Gp36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTS 8; OTS8; PA2.26 antigen; PDPN; podoplanin; RANDAM-2; T1A; T1A-2; T1-alpha
全称	Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A
反应种属	Mouse
应用	Western Blot(0.25 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse Podoplanin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Podoplanin is observed.
使用方法	Western Blot: 0.25 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10630 (Human); 14726 (Mouse); 54320 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Type I Interferons Direct Gammaherpesvirus Host Colonization PLoS Pathog, 2016;12(5):e1005654. Species: Mouse Sample Type: Whole Tissue Application: IHC OCT-embedded Distinct fibroblast lineages determine dermal architecture in skin development and repair. Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F Nature, 2013;504(7479):277-81. Species: Mouse Sample Type: tissue Application: IHC Frozen Regeneration of alveolar type I and II cells from Scgb1a1-expressing cells following severe pulmonary damage induced by bleomycin and influenza. Authors: Zheng, Dahai, Limmon, Gino V, Yin, Lu, Leung, Nicola H, Yu, Hanry, Chow, Vincent, Chen, Jianzhu PLoS ONE, 2012;7(10):e48451. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Impaired humoral immunity and tolerance in K14-VEGFR-3-Ig mice that lack dermal lymphatic drainage. J. Immunol., 2012;189(5):2181-90. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen The Appearance and Modulation of Osteocyte Marker Expression during Calcification of Vascular Smooth Muscle Cells. Authors: Zhu D, Mackenzie NC, Millan JL, Farquharson C, MacRae VE PLoS ONE, 2011;6(5):e19595. Species: Mouse Sample Type: Cell Lysates Application: WB Th17 cells induce ectopic lymphoid follicles in central nervous system tissue inflammation. Authors: Peters A, Pitcher LA, Sullivan JM, Mitsdoerffer M, Acton SE, Franz B, Wucherpfennig K, Turley S, Carroll MC, Sobel RA, Bettelli E, Kuchroo VK Immunity, 2011;35(6):986-96. Species: Mouse Sample Type: In Vivo Application: In vivo Neuropilin-2 mediates VEGF-C-induced lymphatic sprouting together with VEGFR3. Authors: Xu Y, Yuan L, Mak J, Pardanaud L, Caunt M, Kasman I, Larrivee B, Del Toro R, Suchting S, Medvinsky A, Silva J, Yang J, Thomas JL, Koch AW, Alitalo K, Eichmann A, Bagri A J. Cell Biol., 2010;188(1):115-30. Species: Mouse Sample Type: Whole Tissue Application: IHC Fresh
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse Podoplanin Gln21-Lys133 Accession # Q546R8
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Podoplanin, also known as T1 alpha (T1 alpha ), is a mucin type transmembrane glycoprotein with extensive O-glycosylation. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. In addition, non-endothelial cells in numerous normal tissues also express the protein. Within the region used as the immunogen, mouse Podoplanin shares 73.5% and 29% amino acid sequence homology with rat and human Podoplanin, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Stem Cell Markers Endothelial Cell Markers Endothelial Cell Markers and Adhesion Molecules in Angiogenesis ICC / IHC Images: Podoplanin Osteocyte Markers Osteogenesis Markers Special Glycoproteins

参考图片

Detection of Mouse Podoplanin by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Podoplanin at approximately 35-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Podoplanin in Mouse Kidney. Podoplanin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.