Mouse KLF4 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse KLF4 Affinity Purified Polyclonal Ab (25 UG)

货号： AF3158-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3158-SP
别名	Epithelial zinc finger protein EZF; EZF; EZFendothelial Kruppel-like zinc finger protein; GKLFKrueppel-like factor 4; Gut-enriched krueppel-like factor; Kruppel-like factor 4 (gut)
全称	Kruppel-Like Factor 4
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Chromatin Immunoprecipitation (ChIP)(5 µg/5 x 10⁶cells) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects mouse KLF4 in direct ELISAs and Western blots. In these formats, approximately 10% cross-reactivity with recombinant human (rh) KLF4 is observed, and less than 2% cross-reactivity with rhKLF2 and rhKLF5 is observed.
使用方法	Western Blot: 0.1 µg/mL Chromatin Immunoprecipitation (ChIP): 5 µg/5 x 10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	9314 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. HSA21 Single-Minded 2 (Sim2) Binding Sites Co-Localize with Super-Enhancers and Pioneer Transcription Factors in Pluripotent Mouse ES Cells. Authors: Letourneau A, Cobellis G, Fort A, Santoni F, Garieri M, Falconnet E, Ribaux P, Vannier A, Guipponi M, Carninci P, Borel C, Antonarakis S PLoS ONE, 2015;10(5):e0126475. Species: Mouse Sample Type: Whole Cells Application: IP Kruppel-Like Factor 4 Regulates Granule Cell Pax6 Expression and Cell Proliferation in Early Cerebellar Development. Authors: Zhang P, Ha T, Larouche M, Swanson D, Goldowitz D PLoS ONE, 2015;10(7):e0134390. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified STAT3 phosphorylation at tyrosine 705 and serine 727 differentially regulates mouse ESC fates. Authors: Huang G, Yan H, Ye S, Tong C, Ying Q Stem Cells, 2014;32(5):1149-60. Species: Mouse Sample Type: Cell Lysates Application: WB Gbx2, a LIF/Stat3 target, promotes reprogramming to and retention of the pluripotent ground state. Authors: Tai, Chih-I, Ying, Qi-Long J Cell Sci, 2013;126(0):1093-8. Species: Mouse Sample Type: Whole Cells Application: ICC Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embryonic stem cells from refractory mouse strains. Authors: Ye S, Tan L, Yang R, Fang B, Qu S, Schulze EN, Song H, Ying Q, Li P PLoS ONE, 2012;7(4):e35892. Species: Mouse Sample Type: Whole Cells Application: ICC E-cadherin and, in its absence, N-cadherin promotes Nanog expression in mouse embryonic stem cells via STAT3 phosphorylation. Authors: Hawkins K, Mohamet L, Ritson S, Merry C, Ward C Stem Cells, 2012;30(9):1842-51. Species: Mouse Sample Type: Cell Lysates Application: WB Sirt1, p53, and p38(MAPK) Are Crucial Regulators of Detrimental Phenotypes of Embryonic Stem Cells with Max Expression Ablation. Authors: Hishida T, Nozaki Y, Nakachi Y, Mizuno Y, Iseki H, Katano M, Kamon M, Hirasaki M, Nishimoto M, Okazaki Y, Okuda A Stem Cells, 2012;30(8):1634-44. Species: Mouse Sample Type: Cell Lysates Application: WB Heparan sulfation-dependent fibroblast growth factor signaling maintains embryonic stem cells primed for differentiation in a heterogeneous state. Authors: Lanner F, Lee KL, Sohl M, Holmborn K, Yang H, Wilbertz J, Poellinger L, Rossant J, Farnebo F Stem Cells, 2010;28(2):191-200. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse KLF4 Ala11-Phe483 Accession # Q60793
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	KLF4 belongs to the KLF family of transcription factors that regulateseveral cell functions. It is highly expressed in differentiatedepithelial cells of the gastrointestinal tract. Human and mouse KLF4share 91% amino acid sequence homology.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Stem Cell Transcription Factors Embryonic and Induced Pluripotent Stem Cell Markers Endothelial Cell Markers Epithelial Cell Markers and Intracellular Molecules HIF Transcription Factors ICC / IHC Images: KLF4 Kruppel-Like Transcription Factors Neural Stem Cell Transcription Factors Pluripotent Stem Cell Transcription Factors Surface Ectodermal Cells Transcription Factors and Regulators Transcription Factors in the Akt Pathway

参考图片

Detection of KLF4-regulated Genes by Chromatin Immunoprecipitation. D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. KLF4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The B2R promoter was detected by standard PCR.

KLF4 in D3 Mouse Cell Line. KLF4 was detected in immersion fixed D3 mouse embryonic stem cell line using Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.