| 货号 | AF3119-SP |
| 别名 | GLRX; glutaredoxin (thioltransferase); glutaredoxin-1; GRXGRX1; MGC117407; Thioltransferase; thioltransferase-1; TTase-1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects endogenous human and mouse Glutaradoxin in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2745 (Human); 93692 (Mouse); 64045 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cysteine 111 affects aggregation and cytotoxicity of mutant Cu,Zn-superoxide dismutase associated with familial amyotrophic lateral sclerosis. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Glutaredoxin 1 Met1-Leu107 Accession # Q9QUH0 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Glutaredoxin 1s or thioltransferases are a universally distributed family of small molecular weight proteins with thiol-disulfide exchange activity. Glutaredoxin 1 (GLRX1/GRX) is a 12 kDa protein with glutathione-disulfide oxidoreductase activity. Glutaredoxin 1s utilize the reducing power of glutathione to catalyze disulfide reductions in the presence of NADPH and glutathione reductase (the Glutaredoxin 1 system). The amino acid sequence of Glutaredoxin 1 in mouse is highly homologous with that in other mammals (81‑89%), and contains the conserved active sequence motif -Cys-Pro-Try-Cys-. Glutaredoxin 1 also catalyzes the formation of mixed disulfides (glutathionylation), which is an important redox regulatory mechanism, particularly in mammalian cells under oxidative stress conditions, to sense cellular redox potential. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse Glutaredoxin 1 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Glutaredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3119) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Glutaredoxin 1 at approximately 12 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2. |
