| 货号 | AF3109-SP |
| 别名 | MYD88D; myeloid differentiation primary response gene (88); myeloid differentiation primary response protein MyD88 | 全称 | Myeloid Differentiation Primary Response Gene 88 |
| 反应种属 | Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) Immunocytochemistry(5-15 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects mouse and rat MyD88 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL Immunocytochemistry: 5-15 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4615 (Human); 17874 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Toll-like receptor 2-mediated signaling requirements for Francisella tularensis live vaccine strain infection of murine macrophages. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse MyD88 Met1-Pro296 Accession # Q3U7M4 |
| 生物活性 | Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Myeloid Differentiation primary response protein 88 (MyD88) is a 296 amino acid, 34 kDa, ubiquitously expressed, cytoplasmic adaptor protein involved in the signaling of TLR and IL-1 R family members. MyD88 contains an N-terminal death domain and a C-terminal Toll/IL-1 R (TIR) domain. Each domain seems to participate in protein-protein interactions, as the death domain is inactive. Upon Toll receptor ligation, MyD88 is recruited to the receptor and initiates a signaling cascade that results in NK kappa B and JNK activation. The amino acid sequence of mouse MyD88 is 93% identical to that of rat MyD88. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse/Rat MyD88 by Western Blot. Western blot shows lysates of L6 rat myoblast cell line, A20 mouse B cell lymphoma cell line, and CH‑1 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3109) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MyD88 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. | |
MyD88 in RAW 264.7 Mouse Cell Line. MyD88 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse/Rat MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3109) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Rat MyD88 by Simple WesternTM. Simple Western lane view shows lysates of L6 rat myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for MyD88 at approximately 39 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3109) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. | |
Detection of MyD88 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse/Rat MyD88 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3109, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. This application has not been tested in rat samples. |
