| 货号 | AF3059-SP |
| 别名 | 60B8AG; CAGACP-10; Calgranulin A; calgranulin-A; Calprotectin L1L subunit; CFAG; CFAGL1Ag; CGLA; Cystic fibrosis antigen; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP8; MRP-8; MRP8S100 calcium binding protein A8 (calgranulin A); NIF; P8; protein S100-A8; S100 calcium binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A8; Urinary stone protein band A | 全称 | S100 Calcium Binding Protein A8 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.25 µg/mL) Simple Western(2.5 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse S100A8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) S100A9, rmS100A10, and recombinant human S100B is observed. |
| 使用方法 | Western Blot: 0.25 µg/mL Simple Western: 2.5 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6279 (Human); 20201 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Beneficial Effects of Sodium Phenylbutyrate Administration During Infection with Salmonella enterica serovar Typhimurium | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse S100A8 Pro2-Glu89 Accession # P27005 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Mouse S100A8, also known as CP-10, Calgranulin A and MRP8, is a 10kDa member of the S100 family of calcium-binding proteins. S100A8contains short sequential modules, including an N-terminal alpha -helix, a Ca++-bindingEF-hand segment, a short central linker region, a second EF-handsegment, and a C-terminal alpha -helix. S100A8 noncovalently heterodimerizeswith S100A9. In the presence of Ca++, the heterodimers formheterotetramers. The dimeric complex is found both intracellularly andextracellularly. It binds to heparan sulfate and is chemotactic forPMNs and macrophages. The amino acid sequence of mouse S100A8 is 80%and 57% identical to that of rat and human S100A8, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse S100A8 by Western Blot. Western blot shows lysates of mouse spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for S100A8 at approximately 10-11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
S100A8 in NMuMG Mouse Cell Line. S100A8 was detected in immersion fixed NMuMG mouse mammary gland epithelial cell line using 10 µg/mL Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
S100A8 in Mouse Splenocytes. S100A8 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells. | |
Detection of Mouse S100A8 by Simple WesternTM. Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A8 at approximately 9 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
