Mouse FGF-21 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse FGF-21 Affinity Purified Polyclonal Ab (25 UG)

货号： AF3057-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3057-SP
别名	FGF-21; fibroblast growth factor 21
全称	Fibroblast Growth Factor 21
反应种属	Mouse
应用	Western Blot(0.25 µg/mL)
目标/特异性	Detects mouse FGF-21 in direct ELISAs and Western blots. In these formats, approximately 15% cross‑reactivity with recombinant human (rh) FGF-21 is observed and less than 1% cross-reactivity with rhFGF-19 and rhFGF-23 is observed.
使用方法	Western Blot: 0.25 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	26291 (Human); 56636 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Activation of the farnesoid x receptor induces hepatic expression and secretion of fibroblast growth factor 21. Authors: Cyphert HA, Ge X, Kohan AB, Salati LM, Zhang Y, Hillgartner FB J. Biol. Chem., 2012;287(30):25123-38. Species: Rat Sample Type: Cell Lysates Application: WB BetaKlotho is required for metabolic activity of fibroblast growth factor 21. Authors: Ogawa Y, Kurosu H, Yamamoto M, Nandi A, Rosenblatt KP, Goetz R, Eliseenkova AV, Mohammadi M, Kuro-o M Proc. Natl. Acad. Sci. U.S.A., 2007;104(18):7432-7. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse FGF-21 Tyr30-Ser210 Accession # Q9JJN1
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Mouse FGF-21 is a 20 kDa, secreted, nonglycosylated polypeptide that isa member of the FGF family. It is synthesized as a 210 amino acid (aa)precursor that contains a 28 aa signal sequence and a 182 aa matureregion. Mature mouse FGF-21 shares 81%, 80%, 79% and 92% aa sequenceidentity with human, bovine, canine and rat FGF-21, respectively.FGF-21 seems to be produced by the liver and exhibits uniqueantidiabetogenic effects in fat.
运输条件	Blue Ice
存放说明	4℃
参考文献	Diabetes FGF Family FGF Ligands FGF-21: From Cloning to Clinic Glucose Homeostasis Mini-review: Fibroblast Growth Factors (FGFs) Obesity

参考图片

Detection of Mouse FGF‑21 by Western Blot. Western blot shows lysates of mouse spleen tissue and rat spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FGF‑21 Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF3057) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FGF‑21 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.