| 货号 | AF2988-SP |
| 别名 | EC 2.7.10; EC 2.7.10.2; JAK-2; Janus kinase 2Janus kinase 2 (a protein tyrosine kinase); JTK10; tyrosine-protein kinase JAK2 | 全称 | Janus Kinase 2 |
| 反应种属 | Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous mouse and rat Jak2 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3717 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Jak2 Gly2-Val63 Accession # Q62120 |
| 生物活性 | Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Janus Kinase 2 (Jak2) belongs to a family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Activation of Jak2 and other family members Jak1, Jak3, and Tyk2 occurs via phosphorylation at two adjacent tyrosine residues as well as potentially one or more serine residues. Jaks activate members of the STAT family of transcription factors by phosphorylating critical tyrosine regulatory sites. Jak2 plays an essential role in interferon gamma signaling. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse/Rat Jak2 by Western Blot. Western blot shows lysates of DA3 mouse myeloma cell line, PT18 mouse mast/basophil cell line, and L6 rat myoblast cell lines. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Jak2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2988) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Jak2 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
