| 货号 | AF2975-SP |
| 别名 | convertin; EPI; EPITFPI1LACITFI; Extrinsic pathway inhibitor; LACI; Lipoprotein-associated coagulation inhibitor; tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor); tissue factor pathway inhibitor | 全称 | Tissue Factor Pathway Inhibitor |
| 反应种属 | Mouse |
| 应用 | Western Blot(2 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects mouse TFPI in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human TFPI and less than 5% cross-reactivity with recombinant mouse TFPI-2 is observed. |
| 使用方法 | Western Blot: 2 µg/mL Simple Western: 100 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7035 (Human); 21788 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse TFPI Leu29-Lys289 Accession # O54819 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Tissue Factor Pathway Inhibitor (TFPI), also known as lipoprotein-associated coagulation inhibitor (LACI) and extrinsic pathway inhibitor (EPI), is a physiological inhibitor of extrinsic pathway of coagulation and has biological functions of anticoagulation and anti-inflammation (1). It is a secreted protein with an N-terminal acidic region, three Kunitz (K) domains separated by two linker regions, and a C-terminal basic region (2). The first K domain (residues 50-100) inhibits coagulation factor VIIa complexed to tissue factor (TF). The second K domain (residues 121-171) inhibits factor Xa. The third K domain (residues 225-275) binds to heparin (3). The C-terminal basic region may have several functions. For example, it plays an important role in the binding of TFPI to cell surfaces (2). The purified recombinant mouse TFPI ends at residue 289 and does not contain the last 17 residues (residues 290-306) in the C-terminal region. It inhibits the activity of recombinant human Coagulation Factor VII (R&D Systems, Catalog # 2338-SE) in the presence of recombinant human Tissue Factor (Catalog # 2339-PA) . |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of TFPI by Western Blot. Western blot shows lysate of mouse placenta tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse TFPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2975) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TFPI at approximately 37-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
