Mouse Periostin/OSF-2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse Periostin/OSF-2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF2955-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2955-SP
别名	Fasciclin I-like; MGC119510; MGC119511; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52
全称	Osteoblast Specific Factor 2
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects mouse Periostin/OSF‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant rat Periostin/OSF-2 and less than 20% cross-reactivity with recombinant human Periostin/OSF-2 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize Periostin/OSF‑2-mediated adhesion of the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 5 µg/mL Recombinant Mouse Periostin/OSF‑2.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10631 (Human); 50706 (Mouse); 361945 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Macrophage-secreted granulin supports pancreatic cancer metastasis by inducing liver fibrosis Authors: SR Nielsen, V Quaranta, A Linford, P Emeagi, C Rainer, A Santos, L Ireland, T Sakai, K Sakai, YS Kim, D Engle, F Campbell, D Palmer, JH Ko, DA Tuveson, E Hirsch, A Mielgo, MC Schmid Nat Cell Biol, 2016;0(0):. Periostin links mechanical strain to inflammation in abdominal aortic aneurysm. Authors: Yamashita, Osamu, Yoshimura, Koichi, Nagasawa, Ayako, Ueda, Koshiro, Morikage, Noriyasu, Ikeda, Yasuhiro, Hamano, Kimikazu PLoS ONE, 2013;8(11):e79753. Species: Rat Sample Type: Whole Cells Application: Neut Pressure overload-induced cardiac remodeling and dysfunction in the absence of interleukin 6 in mice. Authors: Lai, N Chin, Gao, Mei Hua, Tang, Eric, Tang, Ruoying, Guo, Tracy, Dalton, Nancy D, Deng, Aihua, Tang, Tong Lab Invest, 2012;92(11):1518-26. Species: Mouse Sample Type: Tissue Homogenates Application: WB Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts. Authors: Xu BJ, Yan W, Jovanovic B Proteomics, 2010;10(13):2458-70. Species: Mouse Sample Type: Cell Lysates Application: WB Periostin, a member of a novel family of vitamin K-dependent proteins, is expressed by mesenchymal stromal cells. Authors: Coutu DL, Wu JH, Monette A, Rivard GE, Blostein MD, Galipeau J J. Biol. Chem., 2008;283(26):17991-8001. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Periostin/OSF‑2 Asn24-Gln811 Accession # Q62009
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Mouse Periostin, also known as OSF-2 (osteoblast-specific factor 2) is a 170 kDa, secreted, homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules (1‑4). It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor (2, 5, 6, 7). It is synthesized as a 838 amino acid (aa) precursor that contains a 23 aa signal sequence and an 815 aa mature region (2, 8). It is unknown if the molecule has any significant glycosylation (2). Based on human OSF-2, the homodimer is not disulfide-linked (3). The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while the C-terminus contains four, 130 aa fasciculin type 1 (or FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix (9). Each FAS1 repeat exhibits a novel 7-stranded beta -wedge with a multiple alpha -helix fold (1, 8). Multiple alternate splice forms are known to exist C-terminal (aa 672‑812) to the four-fold FAS1 repeats. These mature molecules are 760, 761, 787 and 788 aa in length and show block deletions of 54 aa, 27 aa and/or 28 aa (10). The significance of the alternate splice forms is not clear. They do, however, appear to be temporally regulated (6). OSF-2 is known to bind to alpha _vbeta₃ and alpha _vbeta₅ integrins (3). It is synthesized by smooth muscle cells, fibroblasts and osteoblasts (2, 5, 7). Mature mouse OSF-2 shares 98%, 92% and 91% aa identity with rat, canine and human OSF‑2, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Clout, N.J. and D. Tisi (2003) Structure 11:197. Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239. Gillan, L. et al. (2002) Cancer Res. 62:5358. Litvin, J. et al. (2005) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 287A:1205. Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77. Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857. Yoshioka, N. et al. (2002) Exp. Cell Res. 279:91. Takeshita, S. et al. (1993) Biochem. J. 294:271. Callebaut, I. et al. (2003) Biochem. Biophys. Res. Commun. 300:619. Swiss-Prot Accession # Q62009.

参考图片

Cell Adhesion Mediated by Periostin/OSF‑2 and Neutralization by Mouse Periostin/
OSF‑2 Antibody. Recombinant Mouse Periostin/OSF‑2 (Catalog # 2955‑F2), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Periostin/OSF‑2 (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Periostin/
OSF‑2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955). The ND₅₀ is typically 1-5 µg/mL.

Periostin/OSF‑2 in Rat Mesenchymal Stem Cells. Periostin/OSF‑2 was detected in immersion fixed rat mesenchymal stem cells differentiated to osteoblasts using Goat Anti-Mouse Periostin/OSF‑2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.