Mouse MICL/CLEC12A Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse MICL/CLEC12A Affinity Purified Polyclonal Ab (25 UG)

货号： AF2950-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2950-SP
别名	CD371; CLEC12A; CLL1; CLL-1; C-type lectin domain family 12, member A; DCAL-2; MICL
全称	Myeloid Inhibitory C-type Lectin-like Receptor
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects recombinant mouse MICL/CLEC12A in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human MICL is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	160364 (Human); 232413 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Clec12a is an inhibitory receptor for uric acid crystals that regulates inflammation in response to cell death. Authors: Neumann K, Castineiras-Vilarino M, Hockendorf U, Hannesschlager N, Lemeer S, Kupka D, Meyermann S, Lech M, Anders H, Kuster B, Busch D, Gewies A, Naumann R, Gross O, Ruland J Immunity, 2014;40(3):389-99. Species: Mouse Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse MICL/CLEC12A Thr67-Arg267 Accession # NP_808354
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	MICL, also known as killer cell lectin-like receptor 1 (KLR1), is an inhibitory C-type lectin-like receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM). It is a75 kDa type II membrane protein that is expressed on neutrophils, NK cells, monocytes and dendritic cells. The amino acid sequence of mouse MICL extracellular domain is 53%, 52% and 58% identical to that of human, bovine and canine MICL, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Stem Cell Markers C-type Lectin Family C-type Lectin Receptors Interactive Pathway: Pathogen or Damage-activated C-Type Lectin Receptor Signaling Pathways ITIM/ITAM Immunoreceptors and Related Molecules

参考图片

Detection of MICL/CLEC12A in M1 Mouse Cell Line by Flow Cytometry. M1 mouse myeloid leukemia cell line was stained with Goat Anti-Mouse MICL/CLEC12A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2950, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

MICL/CLEC12A in Mouse Splenocytes. MICL/CLEC12A was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse MICL/CLEC12A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2950) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.