| 货号 | AF2835-SP |
| 别名 | CD301b | 全称 | Macrophage Galactose-type C-lectin 2 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects mouse MGL2/CD301b in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 1.5-4.5 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated beta ‑Galactosamine-N-Acetyl-Polyacrylamide to immobilized Recombinant Mouse MGL2 (Catalog # 2835-MG) coated at 2.5 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 216864 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. High salt primes a specific activation state of macrophages, M(Na). | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse MGL2/CD301b Ser72-Pro332 Accession # NP_660119 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Mouse Macrophage Galactose N-acetyl-Galactosamine (GalNAc) specific Lectin 2 (MGL2), also known as CD301b, is a 38 kDa member of the C-type lectin family (1). Two MGL proteins are encoded by separate genes in the mouse, but share 91% amino acid (aa) identity in the extracellular domain (ECD) (1). Only one MGL occurs in human and rat and this MGL is structurally more similar to mouse MGL1 than MGL2. However, human MGL and mouse MGL2 both bind specifically to terminal GalNAc residues, in contrast with mouse MGL1 which binds Lewis X (1, 2). GalNAc recognition is likely to be important in dendritic cell-mediated tolerance to self-gangliosides as well as recognition of tumor antigens and parasite glycoproteins (2). Both mouse MGL proteins are expressed on immature dendritic cells. Mouse MGL2 and MGL1 are markers for connective tissue macrophages of a type termed alternately activated macrophages. These macrophages are induced by IL-4 that is produced during Th2-mediated inflammatory responses to parasitic infections or allergic airway inflammation (3, 4). Quantitative RT-PCR after helminth infection shows increasing expression of MGL2 for at least 29 days while MGL1 shows a peak expression at 7 days (3). This, and data from MGL1 knockout mice (5), indicates that MGL1 is critical during formulation of granulation tissue, but MGL2 remains involved during chronic infection. Mouse MGL2 is synthesized as a 332 aa type II transmembrane protein with an N-terminal 51 aa cytoplasmic region, a 26 aa TM segment, and a 255 aa ECD. The ECD contains one 150 aa carbohydrate recognition domain (CRD) that shows 76% and 68% aa identity with rat or human MGL, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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