Targeted deletion of MyD88 in intestinal epithelial cells results in compromised antibacterial immunity associated with downregulation of polymeric immunoglobulin receptor, mucin-2, and antibacterial peptides.
Authors: Frantz A, Rogier E, Weber C, Shen L, Cohen D, Fenton L, Bruno M, Kaetzel C
Mucosal Immunol, 2012;5(5):501-12.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded
Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
Biomark Insights, 2012;7(0):87-104.
Species: Mouse
Sample Type: BALF
Application: WB
Adjuvant effects of ambient particulate matter monitored by proteomics of bronchoalveolar lavage fluid.
Authors: Kang X, Li N, Wang M, Boontheung P, Sioutas C, Harkema JR, Bramble LA, Nel AE, Loo JA
Proteomics, 2010;10(3):520-31.
Species: Mouse
Sample Type: BALF
Application: WB
Efficacy of poly[di(sodium carboxylatophenoxy)phosphazene] (PCPP) as mucosal adjuvant to induce protective immunity against respiratory pathogens.
Authors: Shim DH, Ko HJ, Volker G, Potter AA, Mutwiri G, Babiuk LA, Kweon MN
Vaccine, 2010;28(11):2311-7.
Species: Mouse
Sample Type: BALF
Application: ELISA Development
The member of the cyclic di-nucleotide family bis-(3', 5')-cyclic dimeric inosine monophosphate exerts potent activity as mucosal adjuvant.
Authors: Libanova R, Ebensen T, Schulze K, Bruhn D, Norder M, Yevsa T, Morr M, Guzman CA
Vaccine, 2010;28(10):2249-58.
Species: Mouse
Sample Type: mucosal wash
Application: ELISA Development

The mouse polymeric immunoglobulin receptor (plgR; also known as membrane secretory component) is a 115 kDa type I transmembrane glycoprotein that is synthesized as a 771 amino acid (aa) precursor. It includes an 18 aa signal sequence, a 627 aa extracellular domain (ECD) (aa 19‑645), a 23 aa transmembrane segment (aa 646‑668), and a 143 aa cytoplasmic region (aa 669‑771) (1‑3). The ECD consists of five V-type Ig-like domains and a sixth non-Ig domain that connects to the transmembrane region. The ECD of mouse plgR is 65%, 69%, 85%, 62% and 62% aa identical to the equivalent region in human, porcine, rat, bovine and canine, respectively. plgR is expressed on secretory epithelial cells and serves as a carrier that transports IgA and IgM across epithelium (1, 2, 4). On the basolateral surface of epithelial cells, the receptor initially binds non-covalently to IgA via domains #1 and #5 of the plgR. A rearrangement then occurs where a disulfide bond forms between domain #5 of the plgR and an IgA heavy chain (2). This complex is then internalized and transcytosed to the apical surface. A soluble covalent complex called secretory IgA (SIgA) is generated by proteolytic cleavage of the complex in the sixth extracellular domain of plgR and released into the lumen (5). This proteolytically generated plgR fragment is referred to as secretory component (SC). Notably, in human, plgR transcytoses constitutively, with or without ligand, creating both a bound and free, 78 kDa SC following cleavage (3). In mouse, this event would be expected to generate a 95 kDa fragment (1). The receptor component of the complex anchors the SIgA molecule to mucous (6), where it serves to protect mucous membranes that form a barrier between the interior of the body and the external environment (7).

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2. Piskurich, J. et al. (1995) J. Immunol. 154:1735.
3. Brandtzaeg, P. and F-E. Johansen (2001) Trends Immunol. 22:545.
4. Ben-Hur, H. et al. (2004) Int. J. Mol. Med. 14:35.
5. Asano, M. et al. (2004) Immunology 112:583.
6. Phalipon, A. and B. Corthesy (2003) Trends Immunol. 24:55.
7. Uren, T. et al. (2003) J. Immunol. 170:2531.