Mouse CLEC4F/CLECSF13 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse CLEC4F/CLECSF13 Affinity Purified Polyclonal Ab (25 UG)

货号： AF2784-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2784-SP
别名	CLECSF13; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 13; C-type lectin 13; C-type lectin domain family 4, member F; Galactose Particle Receptor; KCLR; KCR
全称	C-type Lectin Domain Family 4, Member F
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse CLEC4F/CLECSF13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) CLEC4D, rhCLEC4E, and rhCLEC4F is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	165530 (Human); 51811 (Mouse); 114598 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse CLEC4F/CLECSF13 Ala65-Gly548 Accession # P70194
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CLEC4F (C-type lectin domain; family 4, member F; also known as the Kupffer cell receptor and fucose receptor) is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily (1‑3). Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain (ECD) that contains an extended stalk region plus one carbohydrate recognition domain (4, 5). Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation (6, 7). CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues (8). CLEC4F preferentially binds galactose and N‑acetylgalactosamine in a calcium-dependent manner (6, 9, 10). Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system (11).
运输条件	Blue Ice
存放说明	4℃
参考文献	Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179. Bilzer, M. et al. (2006) Liver Int. 26:1175. Kuiper, J. et al. (1994) Biochem. J. 299:285. Accession # P70194. Hoyle, G.W. and R.L. Hill (1988) J. Biol. Chem. 263:7487. Fadden, A.J. et al. (2003) Glycobiology 13:529. Beavil, A.J. et al. (1992) Proc. Natl. Acad. Sci. 89:753. Haltiwanger, R.S. et al. (1986) J. Biol. Chem. 261:7433. Coombs, P.J. et al. (2006) Glycobiology 16:1C. Biessen, E.A.L. et al. (1994) Biochem. J. 299:291. Lehrman, M.A. et al. (1986) J. Biol. Chem. 261:7426.

参考图片

CLEC4F/CLECSF13 in Mouse Liver. CLEC4F/CLECSF13 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse CLEC4F/CLECSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2784) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.