Mouse EPCR Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse EPCR Affinity Purified Polyclonal Ab (25 UG)

货号： AF2749-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2749-SP
别名	APC receptor; CCCA; CCD41; CCD41centrosome-associated protein; CD201 antigen; CD201; centrocyclin; Endothelial cell protein C receptor; endothelial protein C receptor; EPCRMGC23024; PROCR; protein C receptor, endothelial
全称	Endothelial Protein C Receptor
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunoprecipitation(25 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects mouse EPCR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human EPCR is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunoprecipitation: 25 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10544 (Human); 19124 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Vascular immunotargeting to endothelial determinant ICAM-1 enables optimal partnering of recombinant scFv-thrombomodulin fusion with endogenous cofactor. Authors: Greineder, Colin F, Chacko, Ann-Mari, Zaytsev, Sergei, Zern, Blaine J, Carnemolla, Ronald, Hood, Elizabet, Han, Jingyan, Ding, Bi-Sen, Esmon, Charles, Muzykantov, Vladimir PLoS ONE, 2013;8(11):e80110. Species: Mouse Sample Type: Whole Cells Application: ICC Fresh N-cadherin+ HSCs in fetal liver exhibit higher long-term bone marrow reconstitution activity than N-cadherin- HSCs. Biochem Biophys Res Commun, 2012;428(3):354-9. Species: Mouse Sample Type: tissue Application: IHC Frozen Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice. Authors: Xue M, Dervish S, Harrison LC, Fulcher G, Jackson CJ J. Biol. Chem., 2012;287(20):16356-64. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded The Endothelial Protein C Receptor Supports Tissue Factor Ternary Coagulation Initiation Complex Signaling through Protease-activated Receptors. Authors: Disse J, Petersen HH, Larsen KS J. Biol. Chem., 2011;286(7):5756-67. Species: Mouse Sample Type: Cell Lysates Application: WB Activated protein C protects against ventilator-induced pulmonary capillary leak. Authors: Finigan JH, Boueiz A, Wilkinson E, Damico R, Skirball J, Pae HH, Damarla M, Hasan E, Pearse DB, Reddy SP, Grigoryev DN, Cheadle C, Esmon CT, Garcia JG, Hassoun PM Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(6):L1002-11. Species: Mouse Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse EPCR Ala17-Ser214 Accession # Q64695
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Protein C is a vitamin K-dependent serine protease that plays a major role in blood coagulation. Binding of Protein C to EPCR (CD201) leads to the proteolytic activation of PAR1 (protease-activated receptor 1) on endothelial cells and subsequent up-regulation of Protein C-induced genes. EPCR is a type I transmembrane glycoprotein in the CD1/MHC family. It is expressed most strongly in the endothelial cells of arteries and veins in heart and lung. Membrane bound EPCR is released by metalloproteolytic cleavage to generate the soluble receptor. The extracellular domain of human and mouse EPCR shares approximately 61% amino acid sequence homology.
运输条件	Blue Ice
存放说明	4℃
参考文献	Coagulation Endothelial Cell Markers Endothelial Cell Markers and Adhesion Molecules in Angiogenesis Hematopoietic Stem Cell Markers Human CD Marker Products ICC / IHC Images: EPCR Serine Proteases and Regulators

参考图片

Detection of EPCR in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse EPCR Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2749, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

EPCR in bEnd.3 Mouse Cell Line. EPCR was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse EPCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2749) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.