| 货号 | AF2730-SP |
| 别名 | C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 2; CCRG; Colon and small intestine-specific cysteine-rich protein; Colon carcinoma-related gene protein; cysteine-rich secreted A12-alpha-like protein 1; Cysteine-rich secreted protein A12-alpha-like 1; Cysteine-rich secreted protein FIZZ2; FIZZ1; FIZZ2RELM-beta; found in inflammatory zone 1; HXCP2XCP2; RELMb; RELMbeta; resistin like beta; resistin-like beta; RETNL2 | 全称 | Resistin-like Molecule beta |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse RELM beta in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant rat RELM gamma is observed and 10% cross-reactivity with recombinant mouse (rm) Resistin and rmRELM alpha is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 84666 (Human); 57263 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse RELM beta Gly21-Ala105 Accession # Q99P86 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | RELM beta, also known as FIZZ2 (found in inflammatory zone 2) is a 9 kDa secreted, cysteine-rich protein belonging to the RELM family. By analogy to Resistin, RELM beta is expected to exist in two distinct multimeric structures corresponding to hexamers and trimers. RELM beta is synthesized by intestinal goblet cells and is secreted into the intestinal lumen. Mouse RELM beta shares 61% and 77% amino acid (aa) sequence identity with human and rat RELM beta, respectively. It also shares 52%, 71% and 46% aa sequence identity with mouse RELM alpha, RELM gamma and Resistin, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse RELM beta by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse RELM beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2730) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RELM beta at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
