Mouse Nanog Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse Nanog Affinity Purified Polyclonal Ab (25 UG)

货号： AF2729-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2729-SP
别名	FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox
全称	Nanog Homeobox
反应种属	Mouse
应用	Immunocytochemistry(5-15 µg/mL) Intracellular Staining by Flow Cytometry(0.25 µg/10⁶cells)
目标/特异性	Detects recombinant mouse Nanog in Western blots. In this format, approximately 50% cross-reactivity with recombinant human Nanog is observed.
使用方法	Western Blot: Yang, W. et al. (2014) Nat. Comm. 5:3818 Immunocytochemistry: 5-15 µg/mL Intracellular Staining by Flow Cytometry: 0.25 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	79923 (Human); 71950 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Lysine-specific histone demethylase 1 inhibition promotes reprogramming by facilitating the expression of exogenous transcriptional factors and metabolic switch Sci Rep, 2016;6(0):30903. Species: Mouse Sample Type: Whole Cells Application: IHC Fresh ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation Stem Cell Res, 2016;17(2):222-227. Species: Human Sample Type: Whole Cells Application: IHC Not Specified Cyclin-dependent kinase-mediated Sox2 phosphorylation enhances the ability of Sox2 to establish the pluripotent state. Authors: Ouyang J, Yu W, Liu J, Zhang N, Florens L, Chen J, Liu H, Washburn M, Pei D, Xie T J Biol Chem, 2015;290(37):22782-94. Species: Mouse Sample Type: Whole Cells Application: ICC Fluorescence Embryonic Stem Cell Growth Factors Regulate eIF2alpha Phosphorylation. Authors: Friend K, Brooks H, Propson N, Thomson J, Kimble J PLoS ONE, 2015;10(9):e0139076. Species: Mouse Sample Type: Cell Lysates Application: WB Loss of tenomodulin results in reduced self-renewal and augmented senescence of tendon stem/progenitor cells. Authors: Alberton P, Dex S, Popov C, Shukunami C, Schieker M, Docheva D Stem Cells Dev, 2015;24(5):597-609. Species: Mouse Sample Type: Whole Cells Application: IF The histone H2A deubiquitinase Usp16 regulates embryonic stem cell gene expression and lineage commitment. Authors: Yang, Wei, Lee, Yun-Hwa, Jones, Amanda E, Woolnough, Jessica, Zhou, Dewang, Dai, Qian, Wu, Qiang, Giles, Keith E, Townes, Tim M, Wang, Hengbin Nat Commun, 2014;5(0):3818. Species: Mouse Sample Type: Cell Lysates Application: WB STAT3 phosphorylation at tyrosine 705 and serine 727 differentially regulates mouse ESC fates. Authors: Huang G, Yan H, Ye S, Tong C, Ying Q Stem Cells, 2014;32(5):1149-60. Species: Mouse Sample Type: Cell Lysates Application: WB Gbx2, a LIF/Stat3 target, promotes reprogramming to and retention of the pluripotent ground state. Authors: Tai, Chih-I, Ying, Qi-Long J Cell Sci, 2013;126(0):1093-8. Species: Mouse Sample Type: Whole Cells Application: ICC LIF-independent JAK signalling to chromatin in embryonic stem cells uncovered from an adult stem cell disease. Authors: Griffiths DS, Li J, Dawson MA, Trotter MW, Cheng YH, Smith AM, Mansfield W, Liu P, Kouzarides T, Nichols J, Bannister AJ, Green AR, Gottgens B Nat. Cell Biol., 2011;13(1):13-21. Species: Human Sample Type: Whole Cells Application: ICC Small RNA-mediated regulation of iPS cell generation. Authors: Li Z, Yang CS, Nakashima K, Rana TM EMBO J., 2011;30(5):823-34. Species: Mouse Sample Type: Whole Cells Application: ICC Klf5 is involved in self-renewal of mouse embryonic stem cells. Authors: Parisi S, Passaro F, Aloia L, Manabe I, Nagai R, Pastore L, Russo T J. Cell. Sci., 2008;121(0):2629-34. Species: Mouse Sample Type: Cell Lysates Application: ChIP
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse Nanog Trp154-Leu262 Accession # Q80Z64
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells and is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1-3).
运输条件	Blue Ice
存放说明	4℃
参考文献	Mitsui, K. et al. (2003) Cell 11:631. Chambers, I. et al. (2003) Cell 113:643. Hart, A.H. et al. (2004) Dev. Dyn. 230:187.

参考图片

Detection of Nanog in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Goat Anti-Mouse Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2729, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

Nanog in D3 Mouse Cell Line. Nanog was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Mouse Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2729) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.