| 货号 | AF2584-SP |
| 别名 | 10 kDa chaperonin; 10 kDa heat shock protein, mitochondrial; Chaperonin 10 Homolog; Chaperonin 10; CPN10HSP10; Early-pregnancy factor; EPF; GroES; heat shock 10kD protein 1 (chaperonin 10); heat shock 10kDa protein 1 (chaperonin 10); Hsp10; HSPE1 | 全称 | Heat Shock 10 kDa Protein 1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.5 µg/mL) Simple Western(25 µg/mL) |
| 目标/特异性 | Detects mouse HSP10/EPF in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL Simple Western: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3336 (Human); 69253 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse HSP10/EPF Ala2-Asp102 Accession # Q64433 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Protein 10 (HSP10), also known as chaperonin 10 (Cpn10), HSPE1, and Early Pregnancy Factor (EPF), is a 10 kDa heat shock protein that functions within the cell as a molecular chaperone and is associated with HSP60 in the mitochondria. Early pregnancy factor (EPF) has been identified as an extracellular homologue of HSP10. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse HSP10/EPF by Western Blot. Western blot shows lysates of DA3 mouse myeloma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse HSP10/EPF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2584) followed by HRP‑conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HSP10/EPF at approximately 10 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2. | |
Detection of Mouse HSP10/EPF by Simple WesternTM. Simple Western lane view shows lysates of DA3 mouse myeloma cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP10/EPF at approximately 9 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse HSP10/EPF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2584) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
