| 货号 | AF2517-SP |
| 别名 | Glucose-sensitive factor; IDX1; IDX-1GSF; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; Insulin upstream factor 1; IPF1; IPF1pancreas/duodenum homeobox protein 1; Islet/duodenum homeobox-1; MODY4IUF1; pancreatic and duodenal homeobox 1; pancreatic-duodenal homeobox factor 1; PDX-1IPF-1; somatostatin transcription factor 1; Somatostatin-transactivating factor 1; STF-1IUF-1 | 全称 | Pancreas/Duodenum Homeobox-1 |
| 反应种属 | Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse PDX‑1/IPF1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3651 (Human); 18609 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Per-Arnt-Sim kinase regulates pancreatic duodenal homeobox-1 protein stability via phosphorylation of glycogen synthase kinase 3beta in pancreatic beta-cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse PDX‑1/IPF1 Met1-Arg284 Accession # P52946 |
| 生物活性 | Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | PDX-1 (Pancreas-duodenum homeobox 1; also IPF-1, IDX-1, STF-1, GSF and IUF-1) is a 42 kDa member of the IPF-1 subfamily, ANTP homeobox family of proteins. It is expressed in developing pancreas, plus pancreatic beta -cells, and duodenal crypt epithelium. In concert with HNF6, PDX-1 drives pancreatic endocrine cell formation by regulating the expression of neurogenin-3. In adult beta -cells, PDX-1 binds to PCIF-1 to regulate insulin production. Mouse PDX-1 is 284 amino acids (aa) in length (Accession # P52946). It contains one transactivation domain (aa 13‑73) a PBX1 P-P-M-W-K dimerization motif (aa 119‑124), and a DNA-binding homeodomain with an embedded NLS (aa 148‑206). Full-length mouse PDX-1 shares 94% and 88% aa identity with rat and human PDX-1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse/Rat PDX‑1/IPF1 by Western Blot. Western blot shows lysates of beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2517) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDX‑1/IPF1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line. PDX‑1/IPF1 was detected in immersion fixed beta TC‑6 mouse beta cell insulinoma cell line using Goat Anti-Mouse/Rat PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2517) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
PDX‑1/IPF1 in Mouse Pancreas. PDX‑1/IPF1 was detected in perfusion fixed frozen sections of mouse pancreas using Goat Anti-Mouse/Rat PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2517) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in islet cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. | |
Detection of Mouse PDX‑1/IPF1 by Simple WesternTM. Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PDX‑1/IPF1 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2517) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
