| 货号 | AF2504-SP |
| 别名 | CA14; Carbonate dehydratase XIV; carbonic anhydrase XIVcarbonic anhydrase 14; carbonic dehydratase; CAXiV; CA-XIV; EC 4.2.1.1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects mouse Carbonic Anhydrase XIV/CA14 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with rhCA1, -2, -3, -6, -8, -10, -13, recombinant mouse CA4, -9, and -12 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 23632 (Human); 23831 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CO2 chemosensing in rat oesophagus. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase XIV/CA14 Ala16-Met290 Accession # Q9WVT6 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Carbonic anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Mouse CA14 is a type I membrane enzyme highly expressed in the kidney and heart, followed by the skeletal muscle, brain, lung and liver (2). The deduced amino acid sequence of mouse CA14 consists of a signal peptide (residues 1 to 15), an ectodomain (residues 16 to 290), a transmembrane domain (residues 291 to 311) and a cytoplamic region (residues 32 to 337). rmCA14 corresponds to the ectodomain and has activity as described in Activity Assay Protocol. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat Carbonic Anhydrase XIV/CA14 by Western Blot. Western blot shows lysates of human, mouse, and rat brain (cerebellum) tissue and Hepa 1‑6 mouse hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Carbonic Anhydrase XIV/CA14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2504) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase XIV/CA14 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Mouse and Rat Carbonic Anhydrase XIV/CA14 by Simple WesternTM. Simple Western lane view shows lysates of mouse and rat brain (cerebellum) tissue and Hepa 1‑6 mouse hepatoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase XIV/CA14 at approximately 54 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Carbonic Anhydrase XIV/ CA14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2504) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
