Mouse Carbonic Anhydrase IX Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse Carbonic Anhydrase IX Affinity Purified Polyclonal Ab (25 UG)

货号： AF2344-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2344-SP
别名	CA9; CA-IX; CAIXcarbonic anhydrase 9; Carbonate dehydratase IX; carbonic anhydrase IXpMW1; carbonic dehydratase; EC 4.2.1.1; G250; Membrane antigen MN; MN; MNRCC-associated antigen G250; P54/58N; RCC; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL)
目标/特异性	Detects mouse Carbonic Anhydrase IX/CA9 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) CA9 is observed, 5% cross-reactivity with recombinant mouse (rm) CA14 is observed, and less than 1% cross‑reactivity with rhCA1, -2, -3, -8, -10, -13, rmCA4, and -12 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	768 (Human); 230099 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Simultaneous targeted activation of Notch1 and Vhl-disruption in the kidney proximal epithelial tubular cells in mice Sci Rep, 2016;6(0):30739. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Bone marrow-derived mesenchymal stem cells drive lymphangiogenesis. Authors: Maertens L, Erpicum C, Detry B, Blacher S, Lenoir B, Carnet O, Pequeux C, Cataldo D, Lecomte J, Paupert J, Noel A PLoS ONE, 2014;9(9):e106976. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Characterization of the adverse effects of nicotine on placental development: in vivo and in vitro studies. Authors: Holloway, A C, Salomon, A, Soares, M J, Garnier, V, Raha, S, Sergent, F, Nicholson, C J, Feige, J J, Benharouga, M, Alfaidy, N Am J Physiol Endocrinol Metab, 2014;306(4):E443-56. Species: Rat Sample Type: tissue homogenate supernatant Application: WB Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G Nature, 2011;475(7355):226-30. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Cooperation between Pik3ca and p53 mutations in mouse mammary tumor formation. Authors: Adams JR, Xu K, Liu JC, Agamez NM, Loch AJ, Wong RG, Wang W, Wright KL, Lane TF, Zacksenhaus E, Egan SE Cancer Res., 2011;71(7):2706-17. Species: Mouse Sample Type: Tissue Homogenates Application: WB VHL loss actuates a HIF-independent senescence programme mediated by Rb and p400. Authors: Young AP, Schlisio S, Minamishima YA, Zhang Q, Li L, Grisanzio C, Signoretti S, Kaelin WG Nat. Cell Biol., 2008;10(3):361-9. Species: Mouse Sample Type: Whole Tissue Application: IHC CO2 chemosensing in rat oesophagus. Authors: Akiba Y, Mizumori M, Kuo M, Ham M, Guth PH, Engel E, Kaunitz JD Gut, 2008;57(12):1654-64. Species: Rat Sample Type: Whole Cells Application: ICC Carbonic anhydrases and mucosal vanilloid receptors help mediate the hyperemic response to luminal CO2 in rat duodenum. Authors: Akiba Y, Ghayouri S, Takeuchi T, Mizumori M, Guth PH, Engel E, Swenson ER, Kaunitz JD Gastroenterology, 2006;131(1):142-52. Species: Rat Sample Type: Whole Tissue Application: IHC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase IX/CA9 Gln32-Asp390 Accession # Q8VHB5
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20-25, 2003, in Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. Carbonic Anhydrase IX (CA9), also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (2, 3). Recombinant mouse CA9 corresponds to the extracellular portion of mouse CA9.
运输条件	Blue Ice
存放说明	4℃
参考文献	Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.

参考图片

Carbonic Anhydrase IX in Mouse Colon. Carbonic Anhydrase IX was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse Carbonic Anhydrase IX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2344) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the brush border of epithelial cells in villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.