Mouse Chemerin Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Mouse Chemerin Affinity Purified Polyclonal Ab (25 UG)

货号： AF2325-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2325-SP
别名	chemerin; RARRES2; RAR-responsive protein TIG2; retinoic acid receptor responder (tazarotene induced) 2; retinoic acid receptor responder protein 2; Tazarotene-induced gene 2 protein; TIG-2; TIG2HP10433
全称	Retinoic Acid Receptor Responder Protein 2
反应种属	Mouse
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects mouse Chemerin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human Chemerin is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize Chemerin-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human Chem R23. The Neutralization Dose (ND₅₀) is typically 3-9 µg/mL in the presence of 100 ng/mL Recombinant Mouse Chemerin.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5919 (Human); 71660 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Targeting VEGF-A in myeloid cells enhances natural killer cell responses to chemotherapy and ameliorates cachexia Nat Commun, 2016;7(0):12528. Species: Mouse Sample Type: In Vivo Application: Neut Pro- and Anti-Inflammatory Role of ChemR23 Signaling in Pollutant-Induced Inflammatory Lung Responses. Authors: Provoost S, De Grove K, Fraser G, Lannoy V, Tournoy K, Brusselle G, Maes T, Joos G J Immunol, 2016;196(4):1882-90. Species: Mouse Sample Type: Whole Tissue Application: IHC - Paraffin embedded Chemerin neutralization blocks hematopoietic stem cell osteoclastogenesis. Authors: Muruganandan S, Dranse H, Rourke J, McMullen N, Sinal C Stem Cells, 2013;31(10):2172-82. Species: Mouse Sample Type: Cell Lysates Application: WB Chemerin, a novel peroxisome proliferator-activated receptor gamma (PPARgamma) target gene that promotes mesenchymal stem cell adipogenesis. Authors: Muruganandan S, Parlee SD, Rourke JL, Ernst MC, Goralski KB, Sinal CJ J. Biol. Chem., 2011;286(27):23982-95. Species: Mouse Sample Type: Cell Lysates Application: WB The Role of ChemR23 in the Induction and Resolution of Cigarette Smoke-Induced Inflammation. Authors: Demoor T, Bracke KR, Dupont LL J. Immunol., 2011;186(9):5457-67. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Chemerin peptides promote phagocytosis in a ChemR23- and Syk-dependent manner. Authors: Cash JL, Christian AR, Greaves DR J. Immunol., 2010;184(9):5315-24. Species: Mouse Sample Type: In Vivo Application: In vivo Mouse ChemR23 is expressed in dendritic cell subsets and macrophages, and mediates an anti-inflammatory activity of chemerin in a lung disease model. Authors: Luangsay S, Wittamer V, Bondue B, De Henau O, Rouger L, Brait M, Franssen JD, de Nadai P, Huaux F, Parmentier M J. Immunol., 2009;183(10):6489-99. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse Chemerin Thr17-Ser156 Accession # Q9DD06
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Mouse Chemerin, also known as Tazarotene-induced Gene-2 (TIG2), is a new, but distant member of the cystatin superfamily (1-3). Members of this superfamily contain at least two intrachain disulfide bonds and an alpha -helical structure over a distance of about 100 amino acids (2, 3). Chemerin is synthesized as a 162 amino acid (aa) precursor that contains a hydrophobic N-terminal sequence, an intervening 140 aa cystatin-fold containing domain, and a six aa C-terminal prosegment (4-6). Within the cystatin-fold domain there are three intrachain disulfide bonds that contribute to the characteristic fold (4, 7). The precursor molecule is described as undergoing proteolytic processing at both termini by unknown proteases. The N-terminal 16 residue hydrophobic segment is described as being either a signal sequence or a transmembrane (TM) segment for a type II TM protein (5, 8). In either case it gives rise to a soluble proform that undergoes further processing at the C‑terminus (5). In mouse, the C-terminal six residues are cleaved, giving rise to a monomeric, 16 kDa heparin-binding bioactive molecule (aa 17-156) (5-7). A shorter form has been described in human (7). The activity seems to be concentrated in the nine aa’s preceding the prosegment (aa 148-156). Retention of the prosegment blocks activity (4). The 140 aa mature segment is known to bind to the G-protein coupled receptor termed ChemR23 (5, 7). Binding results in macrophage and immature dendritic cell chemotaxis (5). The distribution of this receptor is limited to immune APCs, and it is assumed that Chemerin is an inflammatory molecule. It is unclear which cells are actually producing Chemerin, but keratinocytes, endothelial cells and osteoclasts are potential candidates (1, 7). Mature mouse Chemerin shares 67%, 84%, and 82% aa sequence identity to human, rat, and hamster Chemerin, respectively (6). There is apparently cross-species activity for the protein (6).
运输条件	Blue Ice
存放说明	4℃
参考文献	Nagpal, S. et al. (1997) J. Invest. Dermatol. 109:91. Storici, P. et al. (1996) Eur. J. Biochem. 238:769. Zanetti, M. (2004) J. Leukoc. Biol. 75:39. Wittamer, V. et al. (2004) J. Biol. Chem. 279:9956. Wittamer, V. et al. (2003) J. Exp. Med. 198:977. Busmann, A. et al. (2004) J. Chromatog. B 811:217. Meder, W. et al. (2003) FEBS Lett. 555:495. Yokoyama-Kobayashi, M. et al. (1999) Gene 228:161.

参考图片

Chemotaxis Induced by Chemerin and Neutralization by Mouse Chemerin Antibody. Recombinant Mouse Chemerin (Catalog # 2325-CM) chemoattracts the BaF3 mouse pro‑B cell line transfected with human Chem R23 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse Chemerin (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Chemerin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2325). The ND₅₀ is typically 3-9 µg/mL.