| 货号 | AF2265-SP |
| 别名 | CD334 antigen; CD334; EC 2.7.10; EC 2.7.10.1; FGFR-4; fibroblast growth factor receptor 4; JTK2hydroxyaryl-protein kinase; MGC20292; protein-tyrosine kinase; TKF; tyrosine kinase related to fibroblast growth factor receptor; tyrosylprotein kinase | 全称 | Fibroblast Growth Factor Receptor 4 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.25 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse FGF R4 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) FGF R4 is observed, 5% cross-reactivity with rhFGF R1 alpha (IIIb), rhFGF R1 alpha (IIIc), and rhFGF R1 beta (IIIb) is observed, and less than 1% cross‑reactivity with recombinant mouse (rm) FGF R2 alpha (IIIb), rmFGF R2 beta (IIIb), rmFGF R2 beta (IIIc), rmFGF R3 alpha (IIIb), rmFGF R3 alpha (IIIc), rmFGF R5, and rmFGF R5 beta is observed. |
| 使用方法 | Western Blot: 0.25 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2264 (Human); 14186 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. FGF15/FGFR4 integrates growth factor signaling with hepatic bile acid metabolism and insulin action. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse FGF R4 Leu19-Asp366 Accession # CAA42551 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | FGF R4 is a transmembrane receptor tyrosine kinase that binds several ligands of the FGF family. It is expressed in the early embryo, and its expression has been suggested to play an important role in the formation of mesoderm and posterior structure (1). Recent studies also indicated that FGF R4 may be used as a marker to distinguish neural stem cells from other precursor cells (2). The extracellular domain of mouse FGF R4 shares 95% and 89% amino acid sequence homology with its rat and human homologs, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse FGF R4 by Western Blot. Western blot shows lysates of mouse heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FGF R4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FGF R4 at approximately 89 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
FGF R4 in Mouse Embryo. FGF R4 was detected in immersion fixed frozen sections of mouse embryo (11.5 d.p.c.) using Goat Anti-Mouse FGF R4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2265) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cartilage. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
