| 货号 | AF2254-SP |
| 别名 | bet; delta and Notch-like epidermal growth factor-related receptor; delta/notch-like EGF repeat containing; delta-notch-like EGF repeat-containing transmembrane; H_NH0150O02.1; UNQ26; WUGSC:H_NH0150O02.1 | 全称 | Delta/Notch-like EGF Repeat-containing Transmembrane |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse DNER in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human DNER is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 92737 (Human); 227325 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A search for factors specifying tonotopy implicates DNER in hair-cell development in the chick's cochlea. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse DNER Ala26-His637 Accession # Q8JZM4 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | DNER, also known as BET, is a type I transmembrane glycoprotein that is specifically expressed on nonaxonal areas of post-mitotic neurons. The protein has an extracellular domain containing ten distinct EGF-like repeats similar to those found on Delta and Notch. Human and mouse DNER share 90% amino acid sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
DNER in Mouse Brain. DNER was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Goat Anti-Mouse DNER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2254) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons and molecular layer. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
