Mouse NKp46/NCR1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse NKp46/NCR1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF2225-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2225-SP
别名	CD335 antigen; CD335; hNKp46; Ly94; LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); Lymphocyte antigen 94 homolog; MAR-1; natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; NCR1; NK cell-activating receptor; NKp46; NKP46FLJ99094; NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Agonist Activity(0.4-2.4 µg/mL)
目标/特异性	Detects mouse NKp46/NCR1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) NKp46 is observed and less than 1% cross-reactivity with rhNKp80 and rhNKp30 is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Agonist Activity: 0.4-2.4 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	9437 (Human); 17086 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Murine liver-resident group 1 innate lymphoid cells regulate optimal priming of anti-viral CD8+ T cells J Leukoc Biol, 2016;0(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC OCT-embedded IL-2 in the tumor microenvironment is necessary for Wiskott-Aldrich syndrome protein deficient NK cells to respond to tumors in vivo Sci Rep, 2016;6(0):30636. Species: Mouse Sample Type: Whole Cells Application: Functional Assay Transcription factor KLF2 regulates homeostatic NK cell proliferation and survival Authors: W Rabacal, SK Pabbisetty, KL Hoek, D Cendron, Y Guo, D Maseda, E Sebzda Proc Natl Acad Sci USA, 2016;0(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC - Frozen Role of CC chemokine receptor 4 in natural killer cell activation during acute cigarette smoke exposure. Authors: Stolberg V, Martin B, Mancuso P, Olszewski M, Freeman C, Curtis J, Chensue S Am J Pathol, 2014;184(2):454-63. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Antigen dependently activated cluster of differentiation 8-positive T cells cause perforin-mediated neurotoxicity in experimental stroke. Authors: Mracsko E, Liesz A, Stojanovic A, Lou W, Osswald M, Zhou W, Karcher S, Winkler F, Martin-Villalba A, Cerwenka A, Veltkamp R J Neurosci, 2014;34(50):16784-95. Species: Mouse Sample Type: Whole Cells Application: Bioassay TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells. Authors: Biroccio A, Cherfils-Vicini J, Augereau A, Pinte S, Bauwens S, Ye J, Simonet T, Horard B, Jamet K, Cervera L, Mendez-Bermudez A, Poncet D, Grataroli R, de Rodenbeeke C, Salvati E, Rizzo A, Zizza P, Ricoul M, Cognet C, Kuilman T, Duret H, Lepinasse F, Marvel J, Verhoeyen E, Cosset F, Peeper D, Smyth M, Londono-Vallejo A, Sabatier L, Picco V, Pages G, Scoazec J, Stoppacciaro A, Leonetti C, Vivier E, Gilson E Nat Cell Biol, 2013;15(7):818-28. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins. Authors: Klezovich-Benard M, Corre JP, Jusforgues-Saklani H, Fiole D, Burjek N, Tournier JN, Goossens PL PLoS Pathog., 2012;8(1):e1002481. Species: Mouse Sample Type: Whole Cells Application: Neut Recognition and killing of human and murine pancreatic beta cells by the NK receptor NKp46. Authors: Gur C, Enk J, Kassem SA, Suissa Y, Magenheim J, Stolovich-Rain M, Nir T, Achdout H, Glaser B, Shapiro J, Naparstek Y, Porgador A, Dor Y, Mandelboim O J. Immunol., 2011;187(6):3096-103. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Distinct phenotype and function of NK cells in the pancreas of nonobese diabetic mice. Authors: Brauner H, Elemans M, Lemos S, Broberger C, Holmberg D, Flodstrom-Tullberg M, Karre K, Hoglund P J. Immunol., 2010;184(5):2272-80. Species: Mouse Sample Type: Whole Cells Application: Functional Assay Flt3 permits survival during infection by rendering dendritic cells competent to activate NK cells. Authors: Eidenschenk C, Crozat K, Krebs P, Arens R, Popkin D, Arnold CN, Blasius AL, Benedict CA, Moresco EM, Xia Y, Beutler B Proc. Natl. Acad. Sci. U.S.A., 2010;107(21):9759-64. Species: Mouse Sample Type: Whole Cells Application: Functional Assay Mouse ChemR23 is expressed in dendritic cell subsets and macrophages, and mediates an anti-inflammatory activity of chemerin in a lung disease model. Authors: Luangsay S, Wittamer V, Bondue B, De Henau O, Rouger L, Brait M, Franssen JD, de Nadai P, Huaux F, Parmentier M J. Immunol., 2009;183(10):6489-99. Species: Mouse Sample Type: Whole Cells Application: Flow Regulation of hierarchical clustering and activation of innate immune cells by dendritic cells. Authors: Kang SJ, Liang HE, Reizis B, Locksley RM Immunity, 2008;29(5):819-33. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen CD11cloB220+ interferon-producing killer dendritic cells are activated natural killer cells. Authors: Vosshenrich CA, Lesjean-Pottier S, Hasan M, Richard-Le Goff O, Corcuff E, Mandelboim O, Di Santo JP J. Exp. Med., 2007;204(11):2569-78. Species: Mouse Sample Type: Whole Cells Application: Flow
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse NKp46/NCR1 Glu22-Asn255 Accession # Q8C567
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. In human systems, the CD56^dimCD16⁺ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56^brightCD16^-) expresses low levels of NKp46 in resting cells, but expression is upregulated by IL-2. Mouse NKp46, also known as MAR-1 (3), is a type I transmembrane protein with two extracellular Ig-like domains. It has a positive charge in its transmembrane domain that permits association with the ITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon RI gamma (4). Studies with neutralizing antibodies indicate that the three NCR are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (5). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (6-8).
运输条件	Blue Ice
存放说明	4℃
参考文献	Moretta, L. and A. Moretta (2004) EMBO J. 23:255. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455. Biassoni, R. et al. (1999) Eur. J. Immunol. 29:1014. Westgaard, I. et al. (2004) J. Leukoc. Biol. PMID 15356098. Pende, D. et al. (1999) J. Exp. Med. 190:1505. Arnon, T. et al. (2004) Blood 103:664. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680. Mandelboim, O. et al. (2001) Nature 409:1055.

参考图片

Mouse NKp46/NCR1 Antibody Induces IFN-gamma Secretion in Activated Mouse NK Cells. Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody induces IFN-gamma secretion in mouse natural killer (NK) cells activated with 25 ng/mL Recombinant Mouse IL-2 (Catalog # 402-ML) and 25 ng/mL Recombinant Mouse IL-12 (Catalog # 419-ML), in a dose-dependent manner, as measured using the Quantikine Mouse IFN-gamma ELISA Kit (Catalog # MIF00). The ED₅₀ for this effect is typically
0.4-2.4 μg/mL.

Detection of NKp46/NCR1 in Mouse DX5/CD49b⁺ Splenocytes by Flow Cytometry. Mouse DX5/CD49b⁺ splenocytes were stained with Goat Anti-Mouse NKp46/NCR1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2225, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).