Mouse CHL-1/L1CAM-2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse CHL-1/L1CAM-2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF2147-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2147-SP
别名	CALL; CALLClose homolog of L1; cell adhesion molecule with homology to L1CAM (close homolog of L1); cell adhesion molecule with homology to L1CAM (close homologue of L1); FLJ44930; L1CAM-2; L1CAM2L1 cell adhesion molecule 2; MGC132578; neural cell adhesion molecule L1-like protein
全称	Cell Adhesion Molecule with Homology to L1CAM
反应种属	Mouse
应用	Western Blot(0.25 µg/mL) Simple Western(2.5 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse CHL‑1/L1CAM‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human CHL-1 is observed.
使用方法	Western Blot: 0.25 µg/mL Simple Western: 2.5 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10752 (Human); 12661 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors. Authors: Bye C, Jonsson M, Bjorklund A, Parish C, Thompson L Proc Natl Acad Sci U S A, 2015;112(15):E1946-55. Species: Mouse Sample Type: Whole Tissue Application: IHC- Not specified An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease. Authors: Kizuka Y, Kitazume S, Fujinawa R, Saito T, Iwata N, Saido T, Nakano M, Yamaguchi Y, Hashimoto Y, Staufenbiel M, Hatsuta H, Murayama S, Manya H, Endo T, Taniguchi N EMBO Mol Med, 2015;7(2):175-89. Species: Mouse Sample Type: Homogenized Tissue Application: WB beta-Site amyloid precursor protein (APP)-cleaving enzyme 1 (BACE1)-deficient mice exhibit a close homolog of L1 (CHL1) loss-of-function phenotype involving axon guidance defects. Authors: Hitt B, Riordan S, Kukreja L, Eimer W, Rajapaksha T, Vassar R J Biol Chem, 2012;287(46):38408-25. Species: Mouse Sample Type: tissue Application: IHC Frozen Secretome protein enrichment identifies physiological BACE1 protease substrates in neurons. Authors: Kuhn PH, Koroniak K, Hogl S, Colombo A, Zeitschel U, Willem M, Volbracht C, Schepers U, Imhof A, Hoffmeister A, Haass C, Rossner S, Brase S, Lichtenthaler SF EMBO J., 2012;31(14):3157-68. Species: Mouse Sample Type: Cell Lysates Application: WB EphB regulates L1 phosphorylation during retinocollicular mapping. Mol. Cell. Neurosci., 2012;50(2):201-10. Species: Mouse Sample Type: Cell Lysates Application: IP CHL1 negatively regulates the proliferation and neuronal differentiation of neural progenitor cells through activation of the ERK1/2 MAPK pathway. Authors: Huang X, Zhu LL, Zhao T Mol. Cell. Neurosci., 2011;46(1):296-307. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Phosphatidylinositol 3-kinase/protein kinase Cdelta activation induces close homolog of adhesion molecule L1 (CHL1) expression in cultured astrocytes. Authors: Wu J, Wrathall JR, Schachner M Glia, 2010;58(3):315-28. Species: Mouse Sample Type: Cell Lysates Application: WB CHL1 promotes Sema3A-induced growth cone collapse and neurite elaboration through a motif required for recruitment of ERM proteins to the plasma membrane. Authors: Schlatter MC, Buhusi M, Wright AG, Maness PF J. Neurochem., 2007;104(3):731-44. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse CHL‑1/L1CAM‑2 Ala25-Gln1043 (Leu227-Gln242 del, Ala243Ser) Accession # BAC30699
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Close homolog of L1 (CHL-1), also known as cell adhesion L1-like (CALL) and L1 cell adhesion molecule 2 (L1CAM-2), belongs to the L1 subfamily of immunoglobulin (Ig) superfamily cell adhesion molecules, which also includes L1, neurofascin and NgCAM-related cell adhesion molecule (NrCAM) (1‑3). These molecules are type I transmembrane proteins that have 6 Ig-like domains and 4‑5 fibronectin type III-like (FNIII) domains in their extracellular regions. They also share a highly conserved cytoplasmic region of approximately 110 amino acid (aa) residues containing an ankyrin-binding site. CHL-1 is expressed as a highly glycosylated 185 kDa transmembrane protein by subpopulations of neurons and glia of the central and peripheral nervous system (4, 5). Ectodomain shedding via the metalloprotease-disintegrin ADAM8 releases 165 kDa and 125 kDa soluble CHL-1 fragments, which can diffuse away to function at distant sites (6). CHL-1 is not capable of homotypic interactions, but an extracellular binding partner of CHL-1 has not been identified (4). Human CHL1has been mapped to chromosome 3p26 and is a candidate gene for 3p^-syndrome characterized by mental impairment (7). A missense CHL1 polymorphism associated increased risk of schizophrenia, has also been reported (8). The functional importance of CHL-1 in the nervous system is also evident in CHL-1 deficient mice, which display behavioral abnormalities and show misguided axons within the hippocampus and olfactory tract (9). Enhanced ectodomain-shedding of CHL-1 is also observed in Wobbler mice, the neurodegenerative mutant mice (6). In vitro, soluble or substrate-coated CHL-1 promotes neurite outgrowth and neuronal survival of both cerebellar and hippocampal neurons. Cell surface CHL-1 interacts with integrins in cisto potentiate integrin-dependent cell migration toward extracellular matrix proteins (10). For this enhanced cell motility, CHL-1 linkage to the actin cytoskeleton via interaction between ankyrin and the CHL-1 cytoplasmic region is required.
运输条件	Blue Ice
存放说明	4℃
参考文献	Moos, M. et al. (1988) Nature 334:701. Holm, J. et al. (1996) Eur. J. Neusci. 8:1613. Wei, M. et al. (1998) Hum. Genet. 103:355. Hillenbrand, R. et al. (1999) Eur. J. Neurosci. 11:813. Liu, Q. et al. (2000) J. Neurosci. 20:7682. Naus, S. et al. (2004) J. Biol. Chem. 279:16083. Angeloni, D. et al. (1999) Am. J. Med. Genet. 86:482. Sakurai, K. et al. (2002) Mol. Psychiatry 7:412. Montag-Sallaz, M. et al. (2002) Mol. Cell. Biol. 22:7967. Buhusi, M. et al. (2003) J. Biol. Chem. 278(27):25024.

参考图片

Detection of Mouse CHL‑1/L1CAM‑2 by Western Blot. Western blot shows lysates of mouse brain stem tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse CHL‑1/L1CAM‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2147) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CHL‑1/L1CAM‑2 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse CHL‑1/L1CAM‑2 by Simple Western^TM. Simple Western lane view shows lysates of mouse brain (cortex) tissue and mouse brain stem tissue, loaded at 0.2 mg/mL. A specific band was detected for CHL‑1/L1CAM‑2 at approximately 196-201 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse CHL‑1/L1CAM‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2147) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.