Mouse Legumain Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse Legumain Affinity Purified Polyclonal Ab (25 UG)

货号： AF2058-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2058-SP
别名	AEP; Asparaginyl Endopeptidase; cysteine protease 1; legumain; LGMN; LGMN1; Protease, cysteine 1; protease, cysteine, 1 (legumain); PRSC1EC 3.4.22.34
反应种属	Mouse
应用	Western Blot(0.2 µg/mL)
目标/特异性	Detects mouse Legumain in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Legumain is observed.
使用方法	Western Blot: 0.2 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5641 (Human); 19141 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea. Authors: Zeeuwen PL, van Vlijmen-Willems IM, Cheng T FASEB J., 2010;24(10):3744-55. Species: Mouse Sample Type: recombinant Legumain Application: WB Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis. Authors: Clerin V, Shih HH, Deng N, Hebert G, Resmini C, Shields KM, Feldman JL, Winkler A, Albert L, Maganti V, Wong A, Paulsen JE, Keith JC, Vlasuk GP, Pittman DD Atherosclerosis, 2008;0(0):. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse Legumain Val18-Tyr435 Accession # O89017
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Legumain is a lysosomal cysteine protease found in all mouse tissues examined, but was particularly abundant in kidney and placenta (1). Legumain plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases (2). Over-expression of Legumain in tumors is significant for invasion/metastasis (3). Also known as asparaginyl endopeptidase, it specifically cleaves peptide bonds with Asn at the P1 position. Nevertheless, it also cleaves peptide bonds with Asp at the P1 position. Auto-activation of pro Legumain involves both types of cleavage, which results in the removal of the pro peptides in both C- and N-termini (4). In addition, Legumain activates pro MMP-2 and processes bacterial antigens for MHC class II presentation and pro thymosin alpha to thymosin alpha ₁ and thymosin alpha ₁₁, two acidic peptides with immunoregulatory properties (5-7).
运输条件	Blue Ice
存放说明	4℃
参考文献	Chen, J.M. et al. (1998) Biochem. J. 335:111. Shirahama-Noda, K. et al. (2003) J. Biol. Chem. 278:33194. Liu, C. et al. (2003) Cancer Res. 63: 2957. Li D.N. et al. (2003) J. Biol. Chem. 278:38980. Chen, J.M. et al. (2001) Biol. Chem. 382:777. Schwarz, G. et al. (2002) Biol. Chem. 383:1813. Sarndeses, C.S. et al. (2003) J. Biol. Chem. 278:13286.

参考图片

Detection of Mouse Legumain/Asparaginyl Endopeptidase by Western Blot. Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and Neuro‑2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse Legumain/Asparaginyl Endopeptidase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2058) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Legumain/Asparaginyl Endopeptidase at approximately 37 and 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.