Mouse Endocan Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse Endocan Affinity Purified Polyclonal Ab (25 UG)

货号： AF1999-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1999-SP
别名	endocan; endothelial cell-specific molecule 1; ESM1; ESM-1; IGFBP-rp6
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse Endocan/ESM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human Endocan is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize Endocan/ESM‑1-mediated adhesion of the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 25 µg/mL Recombinant Mouse Endocan/ESM‑1.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	11082 (Human); 71690 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Integrin beta1 controls VE-cadherin localization and blood vessel stability. Authors: Yamamoto H, Ehling M, Kato K, Kanai K, van Lessen M, Frye M, Zeuschner D, Nakayama M, Vestweber D, Adams R Nat Commun, 2015;6(0):6429. Species: Mouse Sample Type: Whole Tissue Application: IHC Transcriptional profiling of VEGF-A and VEGF-C target genes in lymphatic endothelium reveals endothelial-specific molecule-1 as a novel mediator of lymphangiogenesis. Authors: Shin JW, Huggenberger R, Detmar M Blood, 2008;112(6):2318-26. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse Endocan/ESM-1 Trp20-Arg184 Accession # Q9QYY7
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Endocan (endothelial cell proteoglycan; also known as endothelial-cell specific molecule-1 [ESM-1]), is a 50 kDa, monomeric, secreted, cysteine-rich proteoglycan identified initially in endothelial cells of the kidney and lung (1). Mouse Endocan is synthesized as a 184 amino acid (aa) precursor that contains a 21 aa signal sequence and a 20 kDa, 163 aa mature region (2). The N-terminal 2/3 of the molecule contains 18 cysteine residues and there are no potential N-linked glycosylation sites. Based on human Endocan, there are at least two potential O-linked glycosylation sites, one of which will likely be utilized on Ser at position # 136 of the mature molecule (3). The posttranslational modification is approximately 30 kDa in size. It consists of a single dermatan sulfate chain that contains 4-O sulfated N-acetyl galactosamine with alpha -iduronate. This chain is suggested to bind HGF and contribute to HGF mitogenic activity (4). Mature mouse Endocan is 96% and 74% aa identical to rat and human Endocan, respectively. In human, there is a potential for an alternate splice variant. It shows a deletion of aa’s 82 through 131, a range which would not remove the dermatan sulfate attachment site (4). It is not known if such a splice form exists in mouse. Endocan is expressed by endothelial cells, adipocytes, bronchial epithelium and distal renal tubular epithelium (1, 5, 6). It is upregulated by TNF-alpha and VEGF, (1, 7) and is known to bind to LFA-1 ( alpha _Lbeta₂) on the surface of PBMCs, blocking LFA-1 interaction with ICAM-1 (8). Normal circulating levels of Endocan are approximately 1 ng/mL (6).
运输条件	Blue Ice
存放说明	4℃
参考文献	Lassalle, P. et al. (1996) J. Biol. Chem. 271:20458. Lassalle, P. (1999) Genbank Accession #: Q9QYY7. Bechard, D. et al. (2001) J. Biol. Chem. 276:48341. Aitkenhead, M. et al. (2002) Microvasc. Res. 63:159. Wellner, M. et al. (2003) Horm. Metab. Res. 35:217 Bechard, D. et al. (2000) J. Vasc. Res. 37:417. Tsai, J.C. et al. (2002) J. Vasc. Res. 39:148.

参考图片

Endocan/ESM‑1 in Mouse Embryo. Endocan/ESM‑1 was detected in perfusion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Endocan/ESM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1999) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the retina. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Cell Adhesion Mediated by Endocan/ESM‑1 and Neutralization by Mouse Endocan/ESM‑1 Antibody. Recombinant Mouse Endocan/ESM‑1 (Catalog #
1999‑EC), immobilized onto a microplate, supports the adhesion of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Endocan/ESM‑1 (25 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Endocan/ESM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1999). The ND₅₀ is typically 1‑5 µg/mL.